Delivery of Hypoxia Inducible Heme Oxygenase-1 Gene Using Dexamethasone Conjugated Polyethylenimine for Protection of Cardiomyocytes under Hypoxia
- Authors
- Kim, Hyunjung; Kim, Hyun Ah; Choi, Joon Sig; Lee, Minhyung
- Issue Date
- Apr-2009
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- Dexamethasone; Cardiomyocytes; Heme oxygenase-1; Hypoxia; Polyethylenimine
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.30, no.4, pp.897 - 901
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY
- Volume
- 30
- Number
- 4
- Start Page
- 897
- End Page
- 901
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/177036
- DOI
- 10.5012/bkcs.2009.30.4.897
- ISSN
- 0253-2964
- Abstract
- Heme oxygenase-1 (HO-1) is an anti-inflammatory and anti-apoptotic protein and has been applied to various gene therapy researches. However, constitutive expression of HO-I may induce deleterious side effects. In this research, hypoxia inducible HO-I expression plasmid, pEpo-SV-HO-1, was constructed with the erythropoietin (epo) enhancer and simian virus 40 (SV40) promoter to avoid these unwanted side effects. Dexamethasone conjugated polyethylenimine (PEI-Dexa) was used as a gene carrier. It was previously reported that dexamethasone protected cardiomyocytes from apoptosis under hypoxia. In this research, PEI-Dexa reduced the caspase-3 level in hypoxic H9C2 cardiomyocytes as a derivative of dexamethasone, suggesting that PEI-Dexa is an anti-apoptotic reagent as well as a gene carrier. pEpo-SV-HO-1 was transfected to H9C2 cardiomyocytes using PEI-Dexa and the cells were incubated under normoxia or hypoxia. HO-1 expression was induced in the pEpo-SV-HO-1 transfected cells under hypoxia. In addition, cell viability under hypoxia was higher in the pEpo-SV-HO-1 transfected cells than the pEpo-SV-Luc transfected cells. Also, caspase-3 level was reduced in the pEpo-SV-HO-1 transfected cells under hypoxia. In addition to the anti-apoptotic effect of PEI-Dexa, hypoxia inducible HO-1 expression by pEpo-SV-HO-1 may be helpful to protect cardiomyocytes under hypoxia. Therefore, pEpo-SV-HO-1/PEI-Dexa complex may be useful for ischemic heart disease gene therapy.
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