Genistein이 MG-63 조골세포의 증식과 세포외 기질형성에 미치는 영향

Abstract

We investigated the proliferation and extracellular matrix formation effects of genistein on MG-63 cell, human osteoblast like cell. The cells were treated with 1ՌM, 10ՌM, 100ՌM of genistein for 12 h, 24 h, 36 h, 48 h in order to determine the proliferation rate. The growth rate in control group increased up to 36 hours and then decreased at 48 h. Meanwhile, its rate increased rapidly up to at 24 h and then declined at 48 h by treating genistein. The effective time and concentrations of genistein for MG-63 cell was a 12 h and 10ՌM∼100ՌM, respectively. ALP and OC mRNA, initial bone proliferation marker, were strongly expressed by increasing concentration of genistein. The expression of Matrix metalloproteinase (MMP)-13, a collagenase involved in extracellular matrix degradation, and tissue inhibitor of metalloproteinase (TIMP)-1 elevated at 10ՌM of genistein. Based on the results, genistein may affect the proliferation of osteoblast on MG-63 cell in initial time and increased the mineral deposition in the extra cellular matrix. Thus, genistein can play role in preventing bone loss during the initial osteoblast proliferation step and in promoting bone formation by inhibiting bone resorption which resulted from stimulation of the initial osteoblast proliferation due to the increased expression of ALP, OC, MMP-13 and TIMP-1.