Quantitative analysis of phosphatidylcholines and phosphatidylethanolamines in urine of patients with breast cancer by nanoflow liquid chromatography/tandem mass spectrometry
- Authors
- Kim, Hanna; Min, Hye Kyeong; Kong, Gu; Moon, Myeong Hee
- Issue Date
- Mar-2009
- Publisher
- SPRINGER HEIDELBERG
- Keywords
- Phospholipids; Quantitative analysis; nLC-ESI-MS-MS; Urine; Breast cancer
- Citation
- ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.393, no.6-7, pp.1649 - 1656
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANALYTICAL AND BIOANALYTICAL CHEMISTRY
- Volume
- 393
- Number
- 6-7
- Start Page
- 1649
- End Page
- 1656
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/177186
- DOI
- 10.1007/s00216-009-2621-3
- ISSN
- 1618-2642
- Abstract
- Phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs) from urine of patients with breast cancer were qualitatively and quantitatively analyzed by nanoflow liquid chromatography-electrospray ionization tandem mass spectrometry (nLC-ESI-MS-MS). Urinary phospholipids (PLs) were extracted from three different categories of patients (non-cancer controls and breast cancer patients before and after surgery) by first lyophilizing only 1 mL of urine sample to enrich PLs. Next, nLC-ESI-MS-MS analysis of intact urinary phospholipids was performed, resulting in structural identification of 21 PCs and 12 PEs, followed by quantitative analysis using a multiple standard addition method. This study demonstrated that nLC-ESI-MS-MS can be powerfully utilized for the study of relative changes in the contents and concentration of urinary PCs and PEs from breast cancer patients: total concentration of PCs and PEs of patient sample increased to (144 +/- 9)% and (171 +/- 11)%, respectively, compared to control sample but they decreased significantly following surgery.
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