Photoautotrophic production of macular pigment in a Chlamydomonas reinhardtii strain generated by using DNA-free CRISPR-Cas9 RNP-mediated mutagenesis
DC Field | Value | Language |
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dc.contributor.author | Baek, Kwangryul | - |
dc.contributor.author | Yu, Jihyeon | - |
dc.contributor.author | Jeong, Jooyeon | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.contributor.author | Bae, Sangsu | - |
dc.contributor.author | Jin, EonSeon | - |
dc.date.accessioned | 2021-08-02T13:52:27Z | - |
dc.date.available | 2021-08-02T13:52:27Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2018-03 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/17740 | - |
dc.description.abstract | Lutein and zeaxanthin are dietary carotenoids reported to be protective against age-related macular degeneration. Recently, the green alga Chlamydomonas reinhardtii has received attention as a photosynthetic cell factory, but the potential of this alga for carotenoid production has not yet been evaluated. In this study, we selected the C. reinhardtii CC-4349 strain as the best candidate among seven laboratory strains tested for carotenoid production. A knock-out mutant of the zeaxanthin epoxidase gene induced by preassembled DNA-free CRISPR-Cas9 ribonucleoproteins in the CC-4349 strain had a significantly higher zeaxanthin content (56-fold) and productivity (47-fold) than the wild type without the reduction in lutein level. Furthermore, we produced eggs fortified with lutein (2-fold) and zeaxanthin (2.2-fold) by feeding hens a diet containing the mutant. Our results clearly demonstrate the possibility of cost-effective commercial use of microalgal mutants induced by DNA-free CRISPR-Cas9 ribonucleoproteins in algal biotechnology for the production of high-value products. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Photoautotrophic production of macular pigment in a Chlamydomonas reinhardtii strain generated by using DNA-free CRISPR-Cas9 RNP-mediated mutagenesis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Bae, Sangsu | - |
dc.contributor.affiliatedAuthor | Jin, EonSeon | - |
dc.identifier.doi | 10.1002/bit.26499 | - |
dc.identifier.scopusid | 2-s2.0-85041078024 | - |
dc.identifier.wosid | 000423672800018 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOENGINEERING, v.115, no.3, pp.719 - 728 | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.title | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.volume | 115 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 719 | - |
dc.citation.endPage | 728 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | LUTEIN | - |
dc.subject.keywordPlus | CAROTENOIDS | - |
dc.subject.keywordPlus | MICROALGAE | - |
dc.subject.keywordPlus | ZEAXANTHIN | - |
dc.subject.keywordPlus | XANTHOPHYLLS | - |
dc.subject.keywordPlus | ACCUMULATION | - |
dc.subject.keywordPlus | EGG | - |
dc.subject.keywordAuthor | algal biotechnology | - |
dc.subject.keywordAuthor | Chlamydomonas reinhardtii | - |
dc.subject.keywordAuthor | CRISPR-Cas9 | - |
dc.subject.keywordAuthor | lutein | - |
dc.subject.keywordAuthor | zeaxanthin | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/bit.26499 | - |
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