Photoautotrophic production of macular pigment in a Chlamydomonas reinhardtii strain generated by using DNA-free CRISPR-Cas9 RNP-mediated mutagenesis
- Authors
- Baek, Kwangryul; Yu, Jihyeon; Jeong, Jooyeon; Sim, Sang Jun; Bae, Sangsu; Jin, EonSeon
- Issue Date
- Mar-2018
- Publisher
- WILEY
- Keywords
- algal biotechnology; Chlamydomonas reinhardtii; CRISPR-Cas9; lutein; zeaxanthin
- Citation
- BIOTECHNOLOGY AND BIOENGINEERING, v.115, no.3, pp.719 - 728
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY AND BIOENGINEERING
- Volume
- 115
- Number
- 3
- Start Page
- 719
- End Page
- 728
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/17740
- DOI
- 10.1002/bit.26499
- ISSN
- 0006-3592
- Abstract
- Lutein and zeaxanthin are dietary carotenoids reported to be protective against age-related macular degeneration. Recently, the green alga Chlamydomonas reinhardtii has received attention as a photosynthetic cell factory, but the potential of this alga for carotenoid production has not yet been evaluated. In this study, we selected the C. reinhardtii CC-4349 strain as the best candidate among seven laboratory strains tested for carotenoid production. A knock-out mutant of the zeaxanthin epoxidase gene induced by preassembled DNA-free CRISPR-Cas9 ribonucleoproteins in the CC-4349 strain had a significantly higher zeaxanthin content (56-fold) and productivity (47-fold) than the wild type without the reduction in lutein level. Furthermore, we produced eggs fortified with lutein (2-fold) and zeaxanthin (2.2-fold) by feeding hens a diet containing the mutant. Our results clearly demonstrate the possibility of cost-effective commercial use of microalgal mutants induced by DNA-free CRISPR-Cas9 ribonucleoproteins in algal biotechnology for the production of high-value products.
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