Unrestrictive Identification of Multiple Post-translational Modifications from Tandem Mass Spectrometry Using an Error-tolerant Algorithm Based on an Extended Sequence Tag Approachopen access
- Authors
- Na, Seungjin; Jeong, Jaeho; Park, Heejin; Lee, Kong-Joo; Paek, Eunok
- Issue Date
- Dec-2008
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Citation
- MOLECULAR & CELLULAR PROTEOMICS, v.7, no.12, pp.2452 - 2463
- Indexed
- SCIE
SCOPUS
- Journal Title
- MOLECULAR & CELLULAR PROTEOMICS
- Volume
- 7
- Number
- 12
- Start Page
- 2452
- End Page
- 2463
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/177597
- DOI
- 10.1074/mcp.M800101-MCP200
- ISSN
- 1535-9476
- Abstract
- Identification of post-translational modifications (PTMs) is important to understanding the biological functions of proteins. MS/MS is a useful tool to identify PTMs. Most existing search tools are restricted to take only a few types of PTMs as input. Here we describe a new algorithm, called MODi (pronounced "mod eye"), that rapidly searches for all known types of PTMs at once without limiting a multitude of modified sites in a peptide. MODi introduces the notion of a tag chain, a combination structure made from multiple sequence tags, that effectively localizes modified regions within a spectrum and overcomes de novo sequencing errors common in tag-based approaches. MODi showed its performance competence by identifying various types of PTMs in analysis of PTM-rich proteins such as glyceraldehyde-3-phosphate dehydrogenase and lens protein. We demonstrated that MODi innovatively manages the computational complexity of identifying multiple PTMs in a peptide, which may exist in a greater variety than usually expected. In addition, it is suggested that MODi has great potential to discover novel modifications.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 공과대학 > 서울 컴퓨터소프트웨어학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.