Diversity of Ion Channels in Human Bone Marrow Mesenchymal Stem Cells from Amyotrophic Lateral Sclerosis Patientsopen access
- Authors
- Park, Kyoung Sun; Choi, Mi Ran; Jung, Kyoung Hwa; Kim, SeungHyun; Kim, Hyun Young; Kim, Kyung Suk; Cha, Eun-Jong; Kim, Yangmi; Chai, Young Gyu
- Issue Date
- Dec-2008
- Publisher
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Keywords
- Bone marrow; Stem cells; Functional ion channels; Tetrodotoxin-sensitive Na+ current; Passage-dependency
- Citation
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.12, no.6, pp.337 - 342
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Volume
- 12
- Number
- 6
- Start Page
- 337
- End Page
- 342
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/177644
- DOI
- 10.4196/kjpp.2008.12.6.337
- ISSN
- 1226-4512
- Abstract
- Human bone marrow mesenchymal stem cells (hBM-MSCs) represent a potentially valuable cell type for clinical therapeutic applications. The present study was designed to evaluate the effect of long-term culturing (up to 10(th) passages) of hBM-MSCs from eight individual amyotrophic lateral sclerosis (ALS) patients, focusing on functional ion channels. All hBM-MSCs contain several MSCs markers with no significant differences, whereas the distribution of functional ion channels was shown to be different between cells. Four types of K+ currents, including noise-like Ca+2-activated K+ current (IKCa), a transient outward K+ current (I-to), a delayed rectifier K+ current (IKDR), and an inward-rectifier K+ current (K;,) were heterogeneously present in these cells, and a TTX-sensitive Na+ current (I-Na,I-TTx) was also recorded. In the RT-PCR analysis, Kv1.1, heag1, Kv4.2, Kir2.1, MaxiK, and hNE-Na were detected. In particular, I-Na,I-TTx showed a significant passage-dependent increase. This is the first report showing that functional ion channel profiling depend on the cellular passage of hBM-MSCs
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