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Aromatase is phosphorylated in situ at serine-118open access

Authors
Miller, Todd W.Shin, IncheolKagawa, NorioEvans, Dean B.Waterman, Michael R.Arteaga, Carlos L.
Issue Date
Nov-2008
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Keywords
Aromatase; Phosphorylation; Estrogen synthase; Post-translational modification
Citation
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.112, no.1-3, pp.95 - 101
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume
112
Number
1-3
Start Page
95
End Page
101
URI
https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/177720
DOI
10.1016/j.jsbmb.2008.09.001
ISSN
0960-0760
Abstract
Phosphorylation of the cytochrome P450 aromatase has been proposed as a switch to rapidly modulate enzymatic activity and estrogen biosynthesis. Herein, we demonstrate that aromatase serine-118 is a potential phosphoiylation site in mammalian cells. The amino acid context surrounding 5118 is highly conserved among diverse animal species and suggests that an AGC-like kinase may phosphorylate aromatase. Mutation of 5118 to Ala blocked phosphorylation. Mutation of 5118 to either Ala or Asp destabilized aromatase, indicating an important structural role for S118. The phosphomimetic S118D mutant showed decreased specific enzymatic activity, decreased Vmax, and increased Km, while the S118A phospho-inhibiting mutant showed opposite effects. Our findings suggest that phosphorylation of 5718 may decrease aromatase activity, presenting a mechanism whereby kinase signaling may modulate estrogen production and hormone balance.
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