C57BL/6 마우스에서 알코올성 간 독성에 대한 DSF의 효과

Abstract

The aim of this study was to investigate the effect of disulfiram (tetraethylthiuram disulfide, DSF) on the alcoholic liver diseases in mice. C57BL/6 mice were divided into five groups. Control (normal), ethanol treated, DSF treated, ethanol and DSF treated, DSF treated after ethanol treatment for 3 weeks. We evaluated mice body weight and liver weight. On 1 week, body weight was decreased in ethanol treated mice and the body weight gain was similar in all groups of mice after 2 week. With interests, the body weight was significantly increased after treatment of ethanol and DSF for 3 week. However, in the mice with the treatment of DSF only, there was no change of body weight gain and was similar with that of control group of mice. The liver weight of ethanol treated groups was significantly higher than the other groups. A level of ALT, AST and cholesterol in serum were increased in the ethanol treated mice and the level of cholesterol and LDH were increased in DSF treated mice. By H&E staining, we observed degenerative liver tissue change; periportal lymphocytic infiltration, hepatocytes necrosis, severe imflammatory cell infiltrations in ethanol group, and the liver damage was slightly recovered by the treatment of DSF. These results suggest that drinking ethanol is inducible mouse liver damage and the ethanol-induced liver damage was recovered by the treatment of DSF. (Cancer Prev Res 13, 222-227, 2008)