Suppressed production of pro-inflammatory cytokines by LPS-activated macrophages after treatment with Toxoplasma gondii lysateopen access
- Authors
- Lee, Eun-Jung; Heo, Yoo-Mi; Choi, Jong-Hak; Song, Hyun-Ouk; Ryu, Jae-Sook; Ahn, Myoung-Hee
- Issue Date
- Sep-2008
- Publisher
- KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI
- Keywords
- Toxoplasma gondii; LPS; macrophage; cytokine; toll-like receptor; NF-kappa B
- Citation
- KOREAN JOURNAL OF PARASITOLOGY, v.46, no.3, pp.145 - 151
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- KOREAN JOURNAL OF PARASITOLOGY
- Volume
- 46
- Number
- 3
- Start Page
- 145
- End Page
- 151
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/177972
- DOI
- 10.3347/kjp.2008.46.3.145
- ISSN
- 0023-4001
- Abstract
- During Toxoplasma gondii infection, macrophages, dendritic cells, and neutrophils are important sources of pro-inflammatory cytokines from the host. To counteract the pro-inflammatory activities, T gondii is known to have several mechanisms inducing down-regulation of the host immunity. In the present study, we analyzed the production of pro- and anti-inflammatory cytokines from a human myelomonocytic cell line, THP-1 cells, in response to treatment with T. gondii lysate or lipopolysaccharide (LIPS). Treatment of THP-1 cells with LIPS induced production of IL-12, TNF-alpha, IL-8 and IL-10 Co-treatment of THP-1 cells with T gondii lysate inhibited the LPS-induced IL-12, IL-8 and TNF-alpha expression, but increased the level of IL-10 synergistically. IL-12 and IL-10 production was down-regulated by anti-human toll-like reoeptor (TLR)-2 and TLR4 antibodies. T gondii lysate triggered nuclear factor (NF)-kappa B-dependent IL-8 expression in HEK293 cells transfected with TLR2. It is suggested that immunosuppression induced by T gondii lysate treatment might occur via TLR2-mediated NF-kappa B activation.
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