정상 치은 조직에서 배양된 각화세포에서 Cyclosporine A에 의한 MMP-1, TIMP-1, TGF-β1의 발현에 관한 연구The mRNA expression of MMP-1, TIMP-1, TGF-β1 in gingival keratocytes from gingival hyperplasia induced by cyclosporine A
- Other Titles
- The mRNA expression of MMP-1, TIMP-1, TGF-β1 in gingival keratocytes from gingival hyperplasia induced by cyclosporine A
- Authors
- 강학수; 이재선; 빙정호; 박창주; 임재중; 황경균; 심광섭
- Issue Date
- Aug-2008
- Publisher
- 대한구강악안면외과학회
- Keywords
- MMP-1; TIMP-1; TGF-β1; cyclosporine A; gingival keratinocyte
- Citation
- 대한구강악안면외과학회지, v.34, no.4, pp.405 - 411
- Indexed
- KCI
- Journal Title
- 대한구강악안면외과학회지
- Volume
- 34
- Number
- 4
- Start Page
- 405
- End Page
- 411
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/178022
- ISSN
- 2234-7550
- Abstract
- Cyclosporine A (CsA) is a versatile immunosuppresive agent used to prevent graft rejection syndrome and treat autoimmune disease. One of the major side effects associated with CsA is the abnormal gingival hyperplasia. The purpose of this study was to investigate the relationship between the mRNA expression of the MMP-1, TIMP-1, and TGF-β1 and the concentration of CsA in cultured human gingival keratinocytes. Materials & Methods : Gingival keratocytes were obtained from gingival tissues of 4 healthy donors. The cultured gingival keratocytes were incubated with increasing concentrations of CsA (0-2000 ng/ml) for 24 hours and the expression of MMP-1, TIMP-1, and TGF-β1 were determined by reverse transcription-polymerase chain reaction (RT-PCR). Results : The expressions of MMP-1 and TGF-β1 were not significantly different according to the concentrations of CsA. The expression of TIMP-1 was significantly increased at the CsA concentration of 500 ng/ml. Conclusion : We concluded that the gingival hyperplasia induced by CsA was more related with TIMP-1 than MMP-1 or TGF-β1 on gingival collagen metabolism in patients treated with CsA.
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