골수유래줄기세포에서 분화된 골유사세포에서 β-TCP와 rhBMP-2의 골형성 효과에 관한 연구The effects of β-TCP/rhBMP-2 on bone formation in osteoblast-like cells induced from bone marrow-derived mesenchymal stem cells
- Other Titles
- The effects of β-TCP/rhBMP-2 on bone formation in osteoblast-like cells induced from bone marrow-derived mesenchymal stem cells
- Authors
- 최용수; 황경균; 이재선; 박창주; 심광섭
- Issue Date
- Aug-2008
- Publisher
- 대한구강악안면외과학회
- Keywords
- mesenchymal stem cell; rhBMP -2; β-TCP; differentiated osteoblast-like cell
- Citation
- 대한구강악안면외과학회지, v.34, no.4, pp.419 - 427
- Indexed
- KCI
- Journal Title
- 대한구강악안면외과학회지
- Volume
- 34
- Number
- 4
- Start Page
- 419
- End Page
- 427
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/178044
- ISSN
- 2234-7550
- Abstract
- The present study aimed to investigate the osteogenic potentials of differentiated osteoblast-like cells (DOCs) induced from bone marrow-derived mesenchymal stem cells (MSCs) on β-tricalcium phosphate (β-TCP) with recombinant human bone morphogenetic protein (rhBMP-2) in vitro. Osteoblast differentiation was induced in confluent cultures by adding 100 nM dexamethasone, 10 mM β-glycerophosphate, 50 mM L-ascorbic acid. The Alizarin red S staining and reverse transcriptase-polymerase chain reaction (RT-PCR) were perfomed to examine the mRNA expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN), receptor activator for nuclear factor κB ligand (RANKL), runt-related transcription factor 2 (RUNX2), collagen-Ⅰ (COL-Ⅰ).
There were no significant differences in the osteogenic potentials of DOCs induced from MSCs on β-TCP(+/-). According to the incubation period, there were significant increasing of Alizadin red S staining in the induction 3 weeks. The mRNA expression of ALP, RUNX2, and RANKL were higher in DOCs/β-TCP(-) than DOCs/β-TCP(+). According to rhBMP-2 concentrations, the mRNA expression of BSP was significantly increased in DOCs/β-TCP(+) compared to that of DOCs/β-TCP(-) on rhBMP 10 ng/ml. Our study presented the β-TCP will have the possibility that calcium phosphate directly affect the osteoblastic differentiation of the bone marrowderived MSCs.
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