BAF60a interacts with p53 to recruit the SWI/SNF complexopen access
- Authors
- Oh, Jaehak; Sohn, Dong H.; Ko, Myunggon; Chung, Heekyoung; Jeon, Sung H.; Seong, Rho H.
- Issue Date
- May-2008
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Citation
- JOURNAL OF BIOLOGICAL CHEMISTRY, v.283, no.18, pp.11924 - 11934
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF BIOLOGICAL CHEMISTRY
- Volume
- 283
- Number
- 18
- Start Page
- 11924
- End Page
- 11934
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/178685
- DOI
- 10.1074/jbc.M705401200
- ISSN
- 0021-9258
- Abstract
- To understand the tumor-suppressing mechanism of the SWI/SNF chromatin remodeling complex, we investigated its molecular relationship with p53. Using the pREP4-luc episomal reporter, we first demonstrated that p53 utilizes the chromatin remodeling activity of the SWI/SNF complex to initiate transcription from the chromatin-structured promoter. Among the components of the SWI/SNF complex, we identified BAF60a as a mediator of the interaction with p53 by the yeast two-hybrid assay. p53 directly interacted only with BAF60a, but not with other components of the SWI/SNF complex, such as BRG1, SRG3, SNF5, or BAF57. We found out that multiple residues at the amino acid 108-150 region of BAF60a were involved in the interaction with the tetramerization domain of p53. The N-terminal fragment of BAF60a containing the p53-interacting region as well as small interfering RNA for baf60a inhibited the SWI/SNF complex-mediated transcriptional activity of p53. The uncoupling of p53 with the SWI/SNF complex resulted in the repression of both p53-dependent apoptosis and cell cycle arrest by the regulation of target genes. These results suggest that the SWI/SNF chromatin remodeling complex is involved in the suppression of tumors by the interaction with p53.
- Files in This Item
-
- Appears in
Collections - 서울 의과대학 > 서울 병리학교실 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.