Effect of ionic liquid on the retention and separation behavior of various amino acids in reversed phase chromatography
DC Field | Value | Language |
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dc.contributor.author | Yoo, Chang Geun | - |
dc.contributor.author | Yi, Sung Chul | - |
dc.contributor.author | Mun, Sungyong | - |
dc.date.accessioned | 2022-12-21T05:10:28Z | - |
dc.date.available | 2022-12-21T05:10:28Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2007-12 | - |
dc.identifier.issn | 1082-6076 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/179230 | - |
dc.description.abstract | The chromatographic retention behaviors of various amino acids were investigated using ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF4]), as a mobile phase additive and a reversed phase C-18 media as a stationary phase. The amino acids investigated include L-tyrosine, N-CBZ-L-tyrosine, L-phenylalanine, N-CBZ-L-phenylalanine, L-methionine, and N-CBZ-L-methionine. The effect of ionic liquid on N-CBZ-L-amino acids was found to be different from that on L-amino acids. As the concentration of ionic liquid in mobile phase increased, the retention factors of L-amino acids showed a decreasing trend, whereas those of N-CBZ-L-amino acids exhibited a minute change. The selectivity between L-amino acid and N-CBZ-L-amino acid was, thus, improved by increasing the content of ionic liquid. In addition, the peak shapes of L-amino acids, if asymmetric, were transformed into symmetric ones by increasing the ionic liquid content. The increase of feed concentration causes the overloaded condition, leading to a decrease in the retention factor. If such effect of feed concentration is coupled with the effect of using ionic liquid, one can achieve a better separation between L-amino acid and N-CBZ-L-amino acid. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS INC | - |
dc.title | Effect of ionic liquid on the retention and separation behavior of various amino acids in reversed phase chromatography | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yi, Sung Chul | - |
dc.contributor.affiliatedAuthor | Mun, Sungyong | - |
dc.identifier.doi | 10.1080/10826070701629838 | - |
dc.identifier.scopusid | 2-s2.0-35148815279 | - |
dc.identifier.wosid | 000250551700003 | - |
dc.identifier.bibliographicCitation | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, v.30, no.20, pp.2989 - 3006 | - |
dc.relation.isPartOf | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | - |
dc.citation.title | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | - |
dc.citation.volume | 30 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 2989 | - |
dc.citation.endPage | 3006 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | MODIFIER | - |
dc.subject.keywordAuthor | ionic liquid | - |
dc.subject.keywordAuthor | RP-HPLC | - |
dc.subject.keywordAuthor | retention behavior | - |
dc.subject.keywordAuthor | 1-butyl-3-methylimidazolium tetrafluoroborate | - |
dc.subject.keywordAuthor | l-amino acid | - |
dc.subject.keywordAuthor | N-CBZ-L-amino acid | - |
dc.identifier.url | https://www.tandfonline.com/doi/full/10.1080/10826070701629838 | - |
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