Effect of ionic liquid on the retention and separation behavior of various amino acids in reversed phase chromatography
- Authors
- Yoo, Chang Geun; Yi, Sung Chul; Mun, Sungyong
- Issue Date
- Dec-2007
- Publisher
- TAYLOR & FRANCIS INC
- Keywords
- ionic liquid; RP-HPLC; retention behavior; 1-butyl-3-methylimidazolium tetrafluoroborate; l-amino acid; N-CBZ-L-amino acid
- Citation
- JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, v.30, no.20, pp.2989 - 3006
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
- Volume
- 30
- Number
- 20
- Start Page
- 2989
- End Page
- 3006
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/179230
- DOI
- 10.1080/10826070701629838
- ISSN
- 1082-6076
- Abstract
- The chromatographic retention behaviors of various amino acids were investigated using ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF4]), as a mobile phase additive and a reversed phase C-18 media as a stationary phase. The amino acids investigated include L-tyrosine, N-CBZ-L-tyrosine, L-phenylalanine, N-CBZ-L-phenylalanine, L-methionine, and N-CBZ-L-methionine. The effect of ionic liquid on N-CBZ-L-amino acids was found to be different from that on L-amino acids. As the concentration of ionic liquid in mobile phase increased, the retention factors of L-amino acids showed a decreasing trend, whereas those of N-CBZ-L-amino acids exhibited a minute change. The selectivity between L-amino acid and N-CBZ-L-amino acid was, thus, improved by increasing the content of ionic liquid. In addition, the peak shapes of L-amino acids, if asymmetric, were transformed into symmetric ones by increasing the ionic liquid content. The increase of feed concentration causes the overloaded condition, leading to a decrease in the retention factor. If such effect of feed concentration is coupled with the effect of using ionic liquid, one can achieve a better separation between L-amino acid and N-CBZ-L-amino acid.
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