Functional expression of ion channels in mesenchymal stem cells derived from umbilical cord vein
DC Field | Value | Language |
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dc.contributor.author | Park, Kyoung Sun | - |
dc.contributor.author | Jung, Kyoung Hwa | - |
dc.contributor.author | Kim, Seung Hyun | - |
dc.contributor.author | Kim, Kyung Suk | - |
dc.contributor.author | Choi, Mi Ran | - |
dc.contributor.author | Kim, Yangmi | - |
dc.contributor.author | Chai, Young Gyu | - |
dc.date.accessioned | 2022-12-21T07:01:15Z | - |
dc.date.available | 2022-12-21T07:01:15Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2007-08 | - |
dc.identifier.issn | 1066-5099 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/179803 | - |
dc.description.abstract | Mesenchymal stem cells have the ability to renew and differentiate into various lineages of mesenchymal tissues. We used undifferentiated human mesenchymal-like stem cells from human umbilical cord vein (hUC-MSCs), a cell line which contains several mesenchymal cell markers. We characterized functional ion channels in cultured hUC-MSCs with whole-cell patch clamp and reverse transcription-polymerase chain reaction (RT-PCR). Three types of outward current were found in these cells: the Ca2+-activated K+ channel (IKCa), a transient outward K+ current (I-to), and a delayed rectifier K+ current (IKDr). IKCa and IKDR were totally suppressed by tetraethylammonium, and IKCa was sensitive to a specific blocker, iberiotoxin. I-to was inhibited by 4-aminopyridine. Another type of inward rectifier K+ current (K-ir) was also detected in approximately 5% of hUC-MSCs. Elevation of external potassium ion concentration increased the K-ir current amplitude and positively shifted its reversal potential. In addition, inward Na+ current (I-Na) was found in these cells (similar to 30%); the current was blocked by tetrodotoxin and verapamil. In the RT-PCR analysis, Kv1.1, Kv4.2, Kv1.4, Kir2.1, heag1, MaxiK, hNE-Na, and TWIK-1 were detected. These results suggested that multiple functional ion channel currents, IKCa, IKDR, I-to, I-Na, and K-ir, are expressed in hUC-MSCs. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Functional expression of ion channels in mesenchymal stem cells derived from umbilical cord vein | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Seung Hyun | - |
dc.identifier.doi | 10.1634/stemcells.2006-0735 | - |
dc.identifier.scopusid | 2-s2.0-34547875766 | - |
dc.identifier.wosid | 000248411000020 | - |
dc.identifier.bibliographicCitation | STEM CELLS, v.25, no.8, pp.2044 - 2052 | - |
dc.relation.isPartOf | STEM CELLS | - |
dc.citation.title | STEM CELLS | - |
dc.citation.volume | 25 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2044 | - |
dc.citation.endPage | 2052 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.subject.keywordPlus | ADULT BONE-MARROW | - |
dc.subject.keywordPlus | NEURONS IN-VITRO | - |
dc.subject.keywordPlus | PROGENITOR CELLS | - |
dc.subject.keywordPlus | K+-CHANNEL | - |
dc.subject.keywordPlus | POTASSIUM CHANNELS | - |
dc.subject.keywordPlus | BLOOD | - |
dc.subject.keywordPlus | CURRENTS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | ETHER | - |
dc.subject.keywordAuthor | mesenchymal stem cells | - |
dc.subject.keywordAuthor | voltage-gated K+ currents | - |
dc.subject.keywordAuthor | tetrodotoxin-sensitive Na+ current | - |
dc.subject.keywordAuthor | umbilical cord vein | - |
dc.identifier.url | https://academic.oup.com/stmcls/article/25/8/2044/6402484 | - |
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