Functional expression of ion channels in mesenchymal stem cells derived from umbilical cord veinopen access
- Authors
- Park, Kyoung Sun; Jung, Kyoung Hwa; Kim, Seung Hyun; Kim, Kyung Suk; Choi, Mi Ran; Kim, Yangmi; Chai, Young Gyu
- Issue Date
- Aug-2007
- Publisher
- WILEY
- Keywords
- mesenchymal stem cells; voltage-gated K+ currents; tetrodotoxin-sensitive Na+ current; umbilical cord vein
- Citation
- STEM CELLS, v.25, no.8, pp.2044 - 2052
- Indexed
- SCIE
SCOPUS
- Journal Title
- STEM CELLS
- Volume
- 25
- Number
- 8
- Start Page
- 2044
- End Page
- 2052
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/179803
- DOI
- 10.1634/stemcells.2006-0735
- ISSN
- 1066-5099
- Abstract
- Mesenchymal stem cells have the ability to renew and differentiate into various lineages of mesenchymal tissues. We used undifferentiated human mesenchymal-like stem cells from human umbilical cord vein (hUC-MSCs), a cell line which contains several mesenchymal cell markers. We characterized functional ion channels in cultured hUC-MSCs with whole-cell patch clamp and reverse transcription-polymerase chain reaction (RT-PCR). Three types of outward current were found in these cells: the Ca2+-activated K+ channel (IKCa), a transient outward K+ current (I-to), and a delayed rectifier K+ current (IKDr). IKCa and IKDR were totally suppressed by tetraethylammonium, and IKCa was sensitive to a specific blocker, iberiotoxin. I-to was inhibited by 4-aminopyridine. Another type of inward rectifier K+ current (K-ir) was also detected in approximately 5% of hUC-MSCs. Elevation of external potassium ion concentration increased the K-ir current amplitude and positively shifted its reversal potential. In addition, inward Na+ current (I-Na) was found in these cells (similar to 30%); the current was blocked by tetrodotoxin and verapamil. In the RT-PCR analysis, Kv1.1, Kv4.2, Kv1.4, Kir2.1, heag1, MaxiK, hNE-Na, and TWIK-1 were detected. These results suggested that multiple functional ion channel currents, IKCa, IKDR, I-to, I-Na, and K-ir, are expressed in hUC-MSCs.
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