Growth of budding yeasts under optical trap

Abstract

Optic tweezer is powerful tool to investigate biologic cells. Of eukaryotic cells, it was poorly documented regarding to optic trapping to manipulate yeasts. In preliminary experiment to explore yeast biology, interferometric optical tweezers was exploited to trap and manipulate budding yeasts. Successfully, several budding yeasts are trapped simultaneously. We found that the budding direction of the daughter cell was almost outward and the daughter cell surrounded by other yeasts grows slowly or fail to grow. Thus it was assumed that neighboring cells around budding yeast may be critical in budding and the growth of daughter cells. This is first report pertaining to the pattern of yeast budding under the optical trap when multiple yeasts were trapped.