Hydrogenosomal activity of Trichomonas vaginalis cultivated under different iron conditions.open access
- Authors
- Kim, Yong-Seok; Song, Hyun-Ouk; Choi, Ik-Hwa; Park, Soon-Jung; Ryu, Jae-Sook
- Issue Date
- Dec-2006
- Citation
- The Korean journal of parasitology, v.44, no.4, pp.373 - 378
- Indexed
- SCOPUS
KCI
- Journal Title
- The Korean journal of parasitology
- Volume
- 44
- Number
- 4
- Start Page
- 373
- End Page
- 378
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180650
- DOI
- 10.3347/kjp.2006.44.4.373
- ISSN
- 0023-4001
- Abstract
- To evaluate whether iron concentration in TYM medium influence on hydrogenosomal enzyme gene expression and hydrogenosomal membrane potential of Trichomonas vaginalis, trophozoites were cultivated in irondepleted, normal and iron-supplemented TYM media. The mRNA of hydrogenosomal enzymes, such as pyruvate ferredoxin oxidoreductase (PFOR), hydrogenase, ferredoxin and malic enzyme, was increased with iron concentrations in T. vaginalis culture media, measured by RT-PCR. Hydrogenosomal membrane potentials measured with DiOC6 also showed similar tendency, e.g. T. vaginalis cultivated in iron-depleted and iron-supplemented media for 3 days showed a significantly reduced and enhanced hydrogenosomal membrane potential compared with that of normal TYM media, respectively. Therefore, it is suggested that iron may regulate hydrogenosomal activity through hydrogenosomal enzyme expression and hydrogenosomal membrane potential.
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Collections - 서울 의과대학 > 서울 환경의생물학교실 > 1. Journal Articles
- 서울 의과대학 > 서울 생화학·분자생물학교실 > 1. Journal Articles
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