Trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase-3 and reducing Mcl-1 expressionopen access
- Authors
- Kang, Jee-Hae; Song, Hyun-Ouk; Ryu, Jae-Sook; Shin, Myeong Heon; Kim, Jung M; Cho, Yun Sang; Alderete, John F; Ahn, Myoung-Hee; Min, Duk-Young
- Issue Date
- Sep-2006
- Publisher
- WILEY
- Keywords
- Trichomonas vaginalis; neutrophil; apoptosis; caspase-3; Mcl-1
- Citation
- PARASITE IMMUNOLOGY, v.28, no.9, pp.439 - 446
- Indexed
- SCIE
SCOPUS
- Journal Title
- PARASITE IMMUNOLOGY
- Volume
- 28
- Number
- 9
- Start Page
- 439
- End Page
- 446
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181026
- DOI
- 10.1111/j.1365-3024.2006.00884.x
- ISSN
- 0141-9838
- Abstract
- Neutrophils are the predominant inflammatory cells found in the vaginal discharge of patients with Trichomonas vaginalis infection. However, it is not known whether neutrophil apoptosis is induced by live T. vaginalis. Therefore, we examined whether T. vaginalis can influence neutrophil apoptosis, and also whether caspase-3 and the Bcl-2 family members are involved in the apoptosis. Thus, human neutrophils were incubated with live T. vaginalis and neutrophil apoptosis was evaluated by Giemsa, annexin V-PI, and DiOC(6) stainings. The neutrophil apoptosis was significantly higher in those incubated with T. vaginalis than in the control group. When trichomonads were pre-treated with mAb to AP65 (adhesin protein), or when trophozoites were separated from neutrophils using a Transwell chamber, neutrophil apoptosis was significantly reduced. The activation of caspase-3 was evident in neutrophils undergoing spontaneous apoptosis but was markedly enhanced during T. vaginalis-induced apoptosis. Moreover, the inhibition of caspase-3 effectively reduced T. vaginalis-induced apoptosis. Trichomonad-induced apoptosis was also associated with reduced expression of the neutrophil anti-apoptotic protein, Mcl-1. These results indicate that T. vaginalis alters Mcl-1 expression and caspase-3 activation, thereby inducing apoptosis of human neutrophils.
- Files in This Item
-
- Appears in
Collections - 서울 의과대학 > 서울 미생물학교실 > 1. Journal Articles
- 서울 의과대학 > 서울 환경의생물학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181026)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.