Poly(ethylene glycol)/poly(epsilon-caprolactone) diblock copolymeric nanoparticles for non-viral gene delivery: The role of charge group and molecular weight in particle formation, cytotoxicity and transfection
DC Field | Value | Language |
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dc.contributor.author | Jang, Jeong Soon | - |
dc.contributor.author | Kim, So Yeon | - |
dc.contributor.author | Lee, Sang Bong | - |
dc.contributor.author | Kim, Kyung Ok | - |
dc.contributor.author | Han, Joong Soo | - |
dc.contributor.author | Lee, Young Moo | - |
dc.date.accessioned | 2022-12-21T11:12:48Z | - |
dc.date.available | 2022-12-21T11:12:48Z | - |
dc.date.created | 2022-09-16 | - |
dc.date.issued | 2006-06 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181399 | - |
dc.description.abstract | Two types of nanoparticles containing pGL3-Control (plasmid DNA) were prepared using nonionic amphiphlic block copolymers and ionic amphiphilic block copolymers containing a terminal cationic group to investigate the effect of charge on the vehicle properties for systemic gene delivery. Metboxy poly(ethylene glycol) (MPEG)/poly(epsilon-caprolactone) (PCL) diblock copolymers were synthesized by the ring-opening polymerizatrion of epsilon-caprolactone in the presence of a catalyst-free MPEG homopolymer. The hydroxy groups of MPEG/PCL block copolymer were then modified into an amine group to synthesize an amine-terminated MPEG/PCL diblock copolymer (AMPEG/PCL). DNA was incorporated into the polymeric nanoparticles by physical entrapment and electrostatic interaction. All nanoparticle samples exhibited spherical structures and although their sizes increased slightly after DNA-loading, they remained less than 160 nm. The AMPEG/PCL nanoparticles exhibited smaller particle sizes than the MPEG/PCL nanoparticles of the same molecular weight after DNA-loading. The optimum mixing ratio of MPEG/PCL and AMPEG/PCL copolymers to DNA ranged from 4:1 to 1:2 depending on the molecular weight of the block copolymer, the composition of MPEG and PCL and terminal amine group. Based on in vitro cytotoxicity tests, the DNA-loaded MPEG/PCL and AMPEG/PCL nanoparticles did not induce any remarkable cytotoxicity against normal human fibroblasts. Transfection efficiencies of DNA-loaded nanoparticles were improved about 3.4-12.9 times under serum conditions. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.title | Poly(ethylene glycol)/poly(epsilon-caprolactone) diblock copolymeric nanoparticles for non-viral gene delivery: The role of charge group and molecular weight in particle formation, cytotoxicity and transfection | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Han, Joong Soo | - |
dc.contributor.affiliatedAuthor | Lee, Young Moo | - |
dc.identifier.doi | 10.1016/j.jconrel.2006.03.021 | - |
dc.identifier.scopusid | 2-s2.0-33745279554 | - |
dc.identifier.wosid | 000239335000010 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CONTROLLED RELEASE, v.113, no.2, pp.173 - 182 | - |
dc.relation.isPartOf | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.title | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.volume | 113 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 173 | - |
dc.citation.endPage | 182 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | POLYION COMPLEX MICELLES | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | BLOCK-COPOLYMER | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | GLYCOL) | - |
dc.subject.keywordPlus | ENCAPSULATION | - |
dc.subject.keywordPlus | INDOMETHACIN | - |
dc.subject.keywordPlus | NANOSPHERES | - |
dc.subject.keywordPlus | MICROPARTICLES | - |
dc.subject.keywordAuthor | diblock copolymers | - |
dc.subject.keywordAuthor | nanoparticles | - |
dc.subject.keywordAuthor | self-assembly | - |
dc.subject.keywordAuthor | complex | - |
dc.subject.keywordAuthor | DNA delivery | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0168365906001659?via%3Dihub | - |
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