Poly(ethylene glycol)/poly(epsilon-caprolactone) diblock copolymeric nanoparticles for non-viral gene delivery: The role of charge group and molecular weight in particle formation, cytotoxicity and transfection
- Authors
- Jang, Jeong Soon; Kim, So Yeon; Lee, Sang Bong; Kim, Kyung Ok; Han, Joong Soo; Lee, Young Moo
- Issue Date
- Jun-2006
- Publisher
- ELSEVIER
- Keywords
- diblock copolymers; nanoparticles; self-assembly; complex; DNA delivery
- Citation
- JOURNAL OF CONTROLLED RELEASE, v.113, no.2, pp.173 - 182
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CONTROLLED RELEASE
- Volume
- 113
- Number
- 2
- Start Page
- 173
- End Page
- 182
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181399
- DOI
- 10.1016/j.jconrel.2006.03.021
- ISSN
- 0168-3659
- Abstract
- Two types of nanoparticles containing pGL3-Control (plasmid DNA) were prepared using nonionic amphiphlic block copolymers and ionic amphiphilic block copolymers containing a terminal cationic group to investigate the effect of charge on the vehicle properties for systemic gene delivery. Metboxy poly(ethylene glycol) (MPEG)/poly(epsilon-caprolactone) (PCL) diblock copolymers were synthesized by the ring-opening polymerizatrion of epsilon-caprolactone in the presence of a catalyst-free MPEG homopolymer. The hydroxy groups of MPEG/PCL block copolymer were then modified into an amine group to synthesize an amine-terminated MPEG/PCL diblock copolymer (AMPEG/PCL). DNA was incorporated into the polymeric nanoparticles by physical entrapment and electrostatic interaction. All nanoparticle samples exhibited spherical structures and although their sizes increased slightly after DNA-loading, they remained less than 160 nm. The AMPEG/PCL nanoparticles exhibited smaller particle sizes than the MPEG/PCL nanoparticles of the same molecular weight after DNA-loading. The optimum mixing ratio of MPEG/PCL and AMPEG/PCL copolymers to DNA ranged from 4:1 to 1:2 depending on the molecular weight of the block copolymer, the composition of MPEG and PCL and terminal amine group. Based on in vitro cytotoxicity tests, the DNA-loaded MPEG/PCL and AMPEG/PCL nanoparticles did not induce any remarkable cytotoxicity against normal human fibroblasts. Transfection efficiencies of DNA-loaded nanoparticles were improved about 3.4-12.9 times under serum conditions.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 공과대학 > 서울 에너지공학과 > 1. Journal Articles
- 서울 의과대학 > 서울 생화학·분자생물학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181399)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.