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High Productivity of t-PA in CHO Cells Using Hypoxia Response Element
- Issue Date
- May-2006
- Publisher
- 한국미생물·생명공학회
- Keywords
- Chinese hamster ovary cell; hypoxia; hypoxiainducible factor-1α; hypoxia response element; immobilizedcels; tisue-type plasminogen activatorTo produce recombinant proteins in a bioreactor; high-density cell culture and imobilization of cells in caps
- Citation
- Journal of Microbiology and Biotechnology, v.16, no.5, pp 695 - 703
- Pages
- 9
- Indexed
- SCOPUS
KCI
- Journal Title
- Journal of Microbiology and Biotechnology
- Volume
- 16
- Number
- 5
- Start Page
- 695
- End Page
- 703
- ISSN
- 1017-7825
1738-8872
- Abstract
- The disolved oxygen level of any cell cultureenvironment has a critical effect on cellular metabolism.Specifically, hypoxia condition decreases cell viability andrecombinant protein productivity. In this work, to developCHO cells producing recombinant protein with high productivity,mamalian expresion vectors containing a human tissue-typeplasminogen activator (t-PA) gene with hypoxia responseelement (HRE) were constructed and stably transfected intoCHO cels. CHO/2HRE-t-PA cells produced 2-folds higherrecombinant t-PA production than CHO/t-PA cells in a Ba2+-alginate imobilized culture, and 16.8-folds in a repeatedbatch culture. In a non-aerated batch culture of suspension-adapted cels, t-PA productivity of CHO/2HRE/t-PA cels was4.2-folds higher than that of CHO/t-PA cells. Our resultsindicate that HRE is a useful tool for the enhancement ofprotein productivity in mamalian cell cultures.
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