Heparin-binding epidermal growth factor (HB-EGF) may improve embryonic development and implantation by increasing vitronectin receptor (integrin alpha nu beta 3) expression in peri-implantation mouse embryosopen access
- Authors
- Lim, Jung An; Lee, Dong Ryul; Song, Haeng-Seok; Kim, Kye-Seong; Yoon, Tae Ki; Gye, Myung Chan; Kim, Moon Kyoo
- Issue Date
- Mar-2006
- Publisher
- SPRINGER/PLENUM PUBLISHERS
- Keywords
- embryonic development; HB-EGF; integrin alpha nu beta 3; implantation; mouse
- Citation
- JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, v.23, no.3, pp.111 - 119
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
- Volume
- 23
- Number
- 3
- Start Page
- 111
- End Page
- 119
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181687
- DOI
- 10.1007/s10815-006-9021-9
- ISSN
- 1058-0468
- Abstract
- Purpose: This study investigated the effects of HB-EGF on expression of integrin alpha nu beta 3 and implantation of embryos. Methods: Two-cell embryos were recovered and cultured with or without 10 ng/mL HB-EGF for 96h. Expression of integrin alpha nu beta 3 in cultured embryos was examined by real time-RT-PCR and immunofluorescence analysis; embryos were cultured with or without HB-EGF, then transferred into the uteri of pseudo-pregnant female mice in order to analyze their implantation rate. Results: HB-EGF improved embryonic hatching and outgrowth during extended culture, and up-regulated expression of integrin alpha nu beta 3 in both the preimplantation embryo and outgrowing blastocyst. Also, integrin alpha nu beta 3 subunits were localized at the pericellular borders and cell-cell contact areas. The number of successful implantation sites of transferred HB-EGF-treated embryos in the uterus was increased when compared to number of implantation sites with non-treated controls. Conclusions: HB-EGF may improve implantation by accelerating expression of integrin alpha nu beta 3 in peri-implantation mouse embryos.
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Collections - 서울 자연과학대학 > 서울 생명과학과 > 1. Journal Articles
- 서울 의생명공학전문대학원 > 서울 의생명과학과 > 1. Journal Articles
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