Expression of four growth factors in recessed extraocular muscles of rabbits

Abstract

BACKGROUND AND OBJECTIVE: The study was designed to determine the temporal expression of insulin-like growth factor (IGF)-I, IGF-II, basic fibroblast factor 2 (bFGF-2), and transforming growth factor beta 1 (TGF-beta 1) in recessed extraocular muscles. MATERIALS AND METHODS: Sixteen eyes of eight rabbits were subjected to conventional 4 mm recession of superior rectus muscles. Two rabbits were untreated as control. The rabbits were killed and their eyes were enucleated at 3 (group 3), 6 (group 6), 24 (group 24), and 72 (group 72) hours after the operation (two rabbits per group), and the expression of IGF-I, IGF-II, bFGF-2, and TGF-beta 1 was immunohistochemically examined. RESULTS: The peak levels of IGF-I, IGF-II, and TGF-beta 1 expression were observed in groups 24, 6, and 3, respectively. However, bFGF-2 was less expressed than the other growth factors in all groups. CONCLUSIONS: IGF-I, IGF-II, bFGF-2, and TGF-beta 1 in regenerating muscle cells were expressed by different kinetics, suggesting a distinct role of each growth factor during wound healing after recession of extraocular muscles.