신경전달물질 방출 저해제 S8877이 신경세포와 PC12 세포의 돌기신장에 미치는 영향

Abstract

We identified S8877, a novel microbial metabolite from Streptomyces spp., to inhibit the release of neurotransmitter form rat hippocampal neuron and PC12 cells. S8877 is an inhibitor of trifiated norepinephrine ([3H]-NE) release in high K+ buffer solution containing ionomycin, indicating that S8877 inhibits neurotransmitter release after the influx of Ca2+ ions. We also examined the effect of S8877 on β-glucuronidase release from guinea pig neurophils and on the neurite extension of rat hippocampal neurons and PC12 cells. As a result, S8877 increased β-glucruonidas release: when treated with 5μg/ml of S8877, which prevented [3H]-NE release, and the inhibition of neurite extension for both rat hippocampal neurons and PC12 cells was observed.