Composite Multicellular Spheroids Containing Fibers with Pores and Epigallocatechin Gallate (EGCG) Coating on the Surface for Enhanced Proliferation of Stem Cells
- Authors
- Lee, Sangmin; Choi, Soomi; Byun, Hayeon; Lee, Jinkyu; Kwon, Hyunseok; Shin, Heungsoo
- Issue Date
- Dec-2022
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- 3D tissues; diffusion limitation; nanofibers; spheroid; tissue engineering
- Citation
- MACROMOLECULAR BIOSCIENCE, v.22, no.12, pp.1 - 12
- Indexed
- SCIE
SCOPUS
- Journal Title
- MACROMOLECULAR BIOSCIENCE
- Volume
- 22
- Number
- 12
- Start Page
- 1
- End Page
- 12
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/185811
- DOI
- 10.1002/mabi.202200195
- ISSN
- 1616-5187
- Abstract
- Multicellular spheroids are formed by strong cell-cell and cell-extracellular matrix interactions and are widely utilized in tissue engineering for therapeutic treatments or ex vivo tissue modeling. However, diffusion of oxygen into the spheroid gradually decreases, forming a necrotic core. In this study, polycaprolactone (PCL) fibers with pores and epigallocatechin gallate (EGCG) coating on their surface to provide a structural framework within the spheroids and investigated their ability to mitigate diffusional limitation and control over the proliferation of human adipose-derived stem cells (hADSCs) is engineered. The DNA content of composite spheroids prepared from fibers and hADSCs decreased in unadjusted cells (1224 +/- 134 ng), in those with fibers with a smooth surface (SF) (1447 +/- 331 ng), and in those EGCG-coated with SF (E-SF) (1437 +/- 289 ng). Cells with fibers with pores on the surface (PF) (2020 +/- 32 ng) and those with EGCG-coated PF (E-PF) (1911 +/- 80 ng) increased after 7 days of culture, with a significantly greater number of proliferating cells (29 +/- 8% and 30 +/- 8%, respectively). These results indicate that physical modification through the formation of pores on the fiber surface alleviates diffusion limitation of composite spheroids, playing a dominant role over chemical modification.
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