Granulocyte colony stimulating factor treatment in non-alcoholic fatty liver disease: beyond marrow cell mobilizationopen access
- Authors
- Nam, Ho Hyun; Jun, Dae Won; Jang, Kiseok; Saeed, Waqar Khalid; Lee, Jai Sun; Kang, Hyeon Tae; Chae, Yeon Ji
- Issue Date
- Nov-2017
- Publisher
- IMPACT JOURNALS LLC
- Keywords
- non-alcoholic fatty liver; granulocyte colony stimulating factor; apoptosis
- Citation
- ONCOTARGET, v.8, no.58, pp.97965 - 97976
- Indexed
- SCIE
SCOPUS
- Journal Title
- ONCOTARGET
- Volume
- 8
- Number
- 58
- Start Page
- 97965
- End Page
- 97976
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/18682
- DOI
- 10.18632/oncotarget.18967
- ISSN
- 1949-2553
- Abstract
- Protective effects of granulocyte colony stimulating factor (G-CSF) in acute liver injury via marrow cell mobilization have been reported in several studies. But exact mode of action and optimal protocol of G-CSF has been still doubt in chronic disease. Here we investigated mode of action and optimization of G-CSF as a treatment for nonalcoholic fatty liver disease (NAFLD). Various doses of conventional G-CSF (30 mu g/kg once weekly, once daily for 5 days, twice weekly) and long acting G-CSF (30 mu g/kg once a month) were evaluated in two kinds of NAFLD animal models to optimize the G-CSF protocol. G-CSF receptor expression highest increased in NAFLD model among various liver diseases compare to control (NAFLD: 14.7 times, alcohol hepatitis: 7.1 times, cirrhosis: 2.4 times, and ischemia reperfusion: 6.8 times). G-CSF treatment reduced intrahepatic fat accumulation, and inflammation in two kinds of NAFLD animal models. G-CSF increased PI3K/Akt expression in hepatocyte as well as decreased apoptotic drive (increased Bcl-2 expression and decreased Bax expression) in animal model. Five day consecutive G-CSF treatment and once a month long acting G-CSF increased marrow derived stem cell marker in peripheral blood. But twice a week conventional G-CSF treatment did not increased CD34+ cell in peripheral blood and liver neither. Not only high dose G-CSF (once daily for 5 days) but also hepatotropic dose G-CSF (twice a week) significantly reduced hepatocyte apoptosis via PI3K and Akt pathway activation without marrow cell mobilization in NAFLD animal model.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 의과대학 > 서울 병리학교실 > 1. Journal Articles
- 서울 의과대학 > 서울 내과학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/18682)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.