Determination of lysophosphatidylcholine using peroxidase-mimic PVP/PtRu nanozyme
DC Field | Value | Language |
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dc.contributor.author | Park, Ji Yeon | - |
dc.contributor.author | Lee, Han Been | - |
dc.contributor.author | Son, Seong Eun | - |
dc.contributor.author | Gupta, Pramod K. | - |
dc.contributor.author | Park, Yosep | - |
dc.contributor.author | Hur, Won | - |
dc.contributor.author | Seong, Gi Hun | - |
dc.date.accessioned | 2023-07-24T10:07:25Z | - |
dc.date.available | 2023-07-24T10:07:25Z | - |
dc.date.created | 2023-03-14 | - |
dc.date.issued | 2023-02 | - |
dc.identifier.issn | 1618-2642 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/187605 | - |
dc.description.abstract | Lysophosphatidylcholine (LPC) can be used as a biomarker for diseases such as cancer, diabetes, atherosclerosis, and sepsis. In this study, we demonstrated the ability of nanozymes to displace the natural derived enzyme in enzyme-based assays for the measurement of LPC. Synthesized polyvinylpyrrolidone-stabilized platinum-ruthenium nanozymes (PVP/PtRu NZs) had a uniform size of 2.48 +/- 0.24 nm and superb peroxidase-mimicking activity. We demonstrated that the nanozymes had high activity over a wide pH and temperature range and high stability after long-term storage. The LPC concentration could be accurately analyzed through the absorbance and fluorescence signals generated by the peroxidation reaction using the synthesized nanozyme with substrates such as 3,3 ',5,5 '-tetramethylbenzidine (TMB) and 10-acetyl-3,7-dihydroxyphenoxazine (AmplifluTM Red). LPC at a concentration of 0-400 mu M was used for the analysis, and the coefficient of determination (R-2) was 0.977, and the limit of detection (LOD) was 23.1 mu M by colorimetric assay. In the fluorometric assay, the R-2 was 0.999, and the LOD was 8.97 mu M. The spiked recovery values for the determination of LPC concentration in human serum samples were 102-115%. Based on these results, we declared that PVP/PtRu NZs had an ability comparable to that of the native enzyme horseradish peroxidase (HRP) in the enzyme-based LPC detection method. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.title | Determination of lysophosphatidylcholine using peroxidase-mimic PVP/PtRu nanozyme | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Seong, Gi Hun | - |
dc.identifier.doi | 10.1007/s00216-023-04590-1 | - |
dc.identifier.scopusid | 2-s2.0-85148072877 | - |
dc.identifier.wosid | 000933667800001 | - |
dc.identifier.bibliographicCitation | ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.415, no.10, pp.1865 - 1876 | - |
dc.relation.isPartOf | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | - |
dc.citation.title | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | - |
dc.citation.volume | 415 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1865 | - |
dc.citation.endPage | 1876 | - |
dc.type.rims | ART | - |
dc.type.docType | Article; Early Access | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | HORSERADISH-PEROXIDASE | - |
dc.subject.keywordPlus | HYDROGEN-PEROXIDE | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | MIMETICS | - |
dc.subject.keywordPlus | MARKER | - |
dc.subject.keywordAuthor | Nanozyme | - |
dc.subject.keywordAuthor | Lysophosphatidylcholine | - |
dc.subject.keywordAuthor | Platinum nanoparticles | - |
dc.subject.keywordAuthor | Ruthenium nanoparticles | - |
dc.subject.keywordAuthor | Peroxidase-like activity | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s00216-023-04590-1 | - |
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