Determination of lysophosphatidylcholine using peroxidase-mimic PVP/PtRu nanozyme
- Authors
- Park, Ji Yeon; Lee, Han Been; Son, Seong Eun; Gupta, Pramod K.; Park, Yosep; Hur, Won; Seong, Gi Hun
- Issue Date
- Feb-2023
- Publisher
- SPRINGER HEIDELBERG
- Keywords
- Nanozyme; Lysophosphatidylcholine; Platinum nanoparticles; Ruthenium nanoparticles; Peroxidase-like activity
- Citation
- ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.415, no.10, pp.1865 - 1876
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANALYTICAL AND BIOANALYTICAL CHEMISTRY
- Volume
- 415
- Number
- 10
- Start Page
- 1865
- End Page
- 1876
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/187605
- DOI
- 10.1007/s00216-023-04590-1
- ISSN
- 1618-2642
- Abstract
- Lysophosphatidylcholine (LPC) can be used as a biomarker for diseases such as cancer, diabetes, atherosclerosis, and sepsis. In this study, we demonstrated the ability of nanozymes to displace the natural derived enzyme in enzyme-based assays for the measurement of LPC. Synthesized polyvinylpyrrolidone-stabilized platinum-ruthenium nanozymes (PVP/PtRu NZs) had a uniform size of 2.48 +/- 0.24 nm and superb peroxidase-mimicking activity. We demonstrated that the nanozymes had high activity over a wide pH and temperature range and high stability after long-term storage. The LPC concentration could be accurately analyzed through the absorbance and fluorescence signals generated by the peroxidation reaction using the synthesized nanozyme with substrates such as 3,3 ',5,5 '-tetramethylbenzidine (TMB) and 10-acetyl-3,7-dihydroxyphenoxazine (AmplifluTM Red). LPC at a concentration of 0-400 mu M was used for the analysis, and the coefficient of determination (R-2) was 0.977, and the limit of detection (LOD) was 23.1 mu M by colorimetric assay. In the fluorometric assay, the R-2 was 0.999, and the LOD was 8.97 mu M. The spiked recovery values for the determination of LPC concentration in human serum samples were 102-115%. Based on these results, we declared that PVP/PtRu NZs had an ability comparable to that of the native enzyme horseradish peroxidase (HRP) in the enzyme-based LPC detection method.
- Files in This Item
-
Go to Link
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/187605)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.