Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Green synthesis of gold nanoparticles using Euphrasia officinalis leaf extract to inhibit lipopolysaccharide-induced inflammation through NF-κB and JAK/STAT pathways in RAW 264.7 macrophagesopen accessGreen synthesis of gold nanoparticles using Euphrasia officinalis leaf extract to inhibit lipopolysaccharide-induced inflammation through NF-kappa B and JAK/STAT pathways in RAW 264.7 macrophages

Other Titles
Green synthesis of gold nanoparticles using Euphrasia officinalis leaf extract to inhibit lipopolysaccharide-induced inflammation through NF-kappa B and JAK/STAT pathways in RAW 264.7 macrophages
Authors
Liu, YingKim, SenghyunKim, Yeon JuPerumalsamy, HaribalanLee, SeungahHwang, EunsonYi, Tae-Hoo
Issue Date
Apr-2019
Publisher
DOVE MEDICAL PRESS LTD
Keywords
gold nanoparticles; Euphrasia officinalis; lipopolysaccharide; LPS; inflammatory; NF-kappa B; JAK/STAT
Citation
INTERNATIONAL JOURNAL OF NANOMEDICINE, v.14, pp.2945 - 2959
Indexed
SCIE
SCOPUS
Journal Title
INTERNATIONAL JOURNAL OF NANOMEDICINE
Volume
14
Start Page
2945
End Page
2959
URI
https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/189828
DOI
10.2147/IJN.S199781
ISSN
1176-9114
Abstract
Background: Gold nanoparticles (AuNPs) have potential applications in the treatment and diagnosis process, which are attributed to their biocompatibility and high efficiency of drug delivery. In the current study, we utilized an extract of Euphrasia o/ficinalis, a traditional folk medicine, to synthesize gold nanoparticles (EO-AuNPs), and investigated their anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Materials and methods: The AuNPs were synthesized from an ethanol extract of E. officinalis leaves and characterized using several analytical techniques. Anti-inflammatory activities of EO-AuNPs were detected by a model of LPS-induced upregulation of inflammatory mediators and cytokines including nitric oxide (NO), inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNF-alpha), IL-1 beta, and IL-6 in RAW 264.7 cells. The activation of nuclear factor (NF)-kappa B and Janus kinase/signal transducer and activators of transcription (JAK/STAT) signaling pathways was investigated by Western blot. Results: The results confirmed the successful synthesis of AuNPs by E. officinalis. Transmission electron microscopy images showed obvious uptake of EO-AuNPs and internalization into intracellular membrane-bound compartments, resembling endosomes and lysosomes by RAW 264.7 cells. Cell viability assays showed that EO-AuNPs exhibited little cytotoxicity in RAW 264.7 cells at 100 mu g/mL concentration after 24 hours. EO-AuNPs significantly suppressed the LPS-induced release of NO, TNF-alpha, IL-1 beta, and IL-6 as well as the expression of the iNOS gene and protein in RAW 264.7 cells. Further experiments demonstrated that pretreatment with EO-AuNPs significantly reduced the phosphorylation and degradation of inhibitor kappa B-alpha and inhibited the nuclear translocation of NF-kappa B p65. In addition, EO-AuNPs suppressed LPS-stimulated inflammation by blocking the activation of JAK/STAT pathway. Conclusion: The synthesized EO-AuNPs showed anti-inflammatory activity in LPS-induced RAW 264.7 cells, suggesting they may be potential candidates for treating inflammatory-mediated diseases.
Files in This Item
Appears in
Collections
ETC > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Perumalsamy, Haribalan photo

Perumalsamy, Haribalan
서울 부총장(서울) (서울 창의융합교육원)
Read more

Altmetrics

Total Views & Downloads

BROWSE