Nanoporous electroporation needle for localized intracellular delivery in deep tissues
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Gyeong Won | - |
dc.contributor.author | Kim, Byeongyeon | - |
dc.contributor.author | Lee, Tae Wook | - |
dc.contributor.author | Yim, Sang-Gu | - |
dc.contributor.author | Chandrasekharan, Ajeesh | - |
dc.contributor.author | Kim, Hyewon | - |
dc.contributor.author | Choi, Sungyoung | - |
dc.contributor.author | Yang, Seung Yun | - |
dc.date.accessioned | 2023-09-26T07:42:18Z | - |
dc.date.available | 2023-09-26T07:42:18Z | - |
dc.date.created | 2023-01-05 | - |
dc.date.issued | 2023-07 | - |
dc.identifier.issn | 2380-6761 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/191095 | - |
dc.description.abstract | The exogenous control of intracellular drug delivery has been shown to improve the overall efficacy of therapies by reducing nonspecific off-target toxicity. However, achieving a precise on-demand dosage of a drug in deep tissues with minimal damage is still a challenge. In this study, we report an electric-pulse-driven nanopore-electroporation (nEP) system for the localized intracellular delivery of a model agent in deep tissues. Compared with conventional bulk electroporation, in vitro nEP achieved better transfection efficiency (>60%) with a high cell recovery rate (>95%) under a nontoxic low electroporation condition (40 V). Furthermore, in vivo nEP using a nanopore needle electrode with a side drug-releasing compartment offered better control over the dosage release, time, and location of propidium iodide, which was used as a model agent for intracellular delivery. In a pilot study using experimental animals, the nEP system exhibited two times higher transfection efficiency of propidium iodide in the thigh muscle tissue, while minimizing tissue damage (<20%) compared to that of bulk electroporation. This tissue-penetrating nEP platform can provide localized, safe, and effective intracellular delivery of diverse therapeutics into deep tissues in a controlled manner. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Nanoporous electroporation needle for localized intracellular delivery in deep tissues | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Sungyoung | - |
dc.identifier.doi | 10.1002/btm2.10418 | - |
dc.identifier.scopusid | 2-s2.0-85144090157 | - |
dc.identifier.wosid | 000895328900001 | - |
dc.identifier.bibliographicCitation | BIOENGINEERING & TRANSLATIONAL MEDICINE, v.8, no.4, pp.1 - 12 | - |
dc.relation.isPartOf | BIOENGINEERING & TRANSLATIONAL MEDICINE | - |
dc.citation.title | BIOENGINEERING & TRANSLATIONAL MEDICINE | - |
dc.citation.volume | 8 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article; Early Access | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | SIRNA DELIVERY | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | GENE-TRANSFER | - |
dc.subject.keywordPlus | ELECTROCHEMOTHERAPY | - |
dc.subject.keywordPlus | TRANSFECTION | - |
dc.subject.keywordPlus | EFFICIENCY | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordAuthor | electric pulse-driven drug delivery | - |
dc.subject.keywordAuthor | electroporation | - |
dc.subject.keywordAuthor | intracellular delivery | - |
dc.subject.keywordAuthor | nanopore membrane | - |
dc.identifier.url | https://aiche.onlinelibrary.wiley.com/doi/10.1002/btm2.10418 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.