Nanoporous electroporation needle for localized intracellular delivery in deep tissuesopen access
- Authors
- Lee, Gyeong Won; Kim, Byeongyeon; Lee, Tae Wook; Yim, Sang-Gu; Chandrasekharan, Ajeesh; Kim, Hyewon; Choi, Sungyoung; Yang, Seung Yun
- Issue Date
- Jul-2023
- Publisher
- WILEY
- Keywords
- electric pulse-driven drug delivery; electroporation; intracellular delivery; nanopore membrane
- Citation
- BIOENGINEERING & TRANSLATIONAL MEDICINE, v.8, no.4, pp.1 - 12
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOENGINEERING & TRANSLATIONAL MEDICINE
- Volume
- 8
- Number
- 4
- Start Page
- 1
- End Page
- 12
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/191095
- DOI
- 10.1002/btm2.10418
- ISSN
- 2380-6761
- Abstract
- The exogenous control of intracellular drug delivery has been shown to improve the overall efficacy of therapies by reducing nonspecific off-target toxicity. However, achieving a precise on-demand dosage of a drug in deep tissues with minimal damage is still a challenge. In this study, we report an electric-pulse-driven nanopore-electroporation (nEP) system for the localized intracellular delivery of a model agent in deep tissues. Compared with conventional bulk electroporation, in vitro nEP achieved better transfection efficiency (>60%) with a high cell recovery rate (>95%) under a nontoxic low electroporation condition (40 V). Furthermore, in vivo nEP using a nanopore needle electrode with a side drug-releasing compartment offered better control over the dosage release, time, and location of propidium iodide, which was used as a model agent for intracellular delivery. In a pilot study using experimental animals, the nEP system exhibited two times higher transfection efficiency of propidium iodide in the thigh muscle tissue, while minimizing tissue damage (<20%) compared to that of bulk electroporation. This tissue-penetrating nEP platform can provide localized, safe, and effective intracellular delivery of diverse therapeutics into deep tissues in a controlled manner.
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