Performance of the Real-Q EBV Quantification Kit for Epstein-Barr Virus DNA Quantification in Whole Blood
DC Field | Value | Language |
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dc.contributor.author | Huh, Hee Jae | - |
dc.contributor.author | Eun, Park Jong | - |
dc.contributor.author | Kim, Ji-Youn | - |
dc.contributor.author | Yun, Sun Ae | - |
dc.contributor.author | Lee, Myoung-Keun | - |
dc.contributor.author | Lee, Nam Yong | - |
dc.contributor.author | Kim, Jong-Won | - |
dc.contributor.author | Ki, Chang-Seok | - |
dc.date.accessioned | 2023-11-14T08:47:13Z | - |
dc.date.available | 2023-11-14T08:47:13Z | - |
dc.date.created | 2023-07-07 | - |
dc.date.issued | 2017-03 | - |
dc.identifier.issn | 2234-3806 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/192389 | - |
dc.description.abstract | There has been increasing interest in standardized and quantitative Epstein-Barr virus (EBV) DNA testing for the management of EBV disease. We evaluated the performance of the Real-Q EBV Quantification Kit (BioSewoom, Korea) in whole blood (WB). Nucleic acid extraction and real-time PCR were performed by using the MagNA Pure 96 (Roche Diagnostics, Germany) and 7500 Fast real-time PCR system (Applied Biosystems, USA), respectively. Assay sensitivity, linearity, and conversion factor were determined by using the World Health Organization international standard diluted in EBV-negative WB. We used 81 WB clinical specimens to compare performance of the Real-Q EBV Quantification Kit and artus EBV RG PCR Kit (Qiagen, Germany). The limit of detection (LOD) and limit of quantification (LOQ) for the Real-Q kit were 453 and 750 IU/mL, respectively. The conversion factor from EBV genomic copies to IU was 0.62. The linear range of the assay was from 750 to 106 IU/mL. Viral load values measured with the Real-Q assay were on average 0.54 log(10) copies/mL higher than those measured with the artus assay. The Real-Q assay offered good analytical performance for EBV DNA quantification in WB. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC LABORATORY MEDICINE | - |
dc.title | Performance of the Real-Q EBV Quantification Kit for Epstein-Barr Virus DNA Quantification in Whole Blood | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Eun, Park Jong | - |
dc.identifier.doi | 10.3343/alm.2017.37.2.147 | - |
dc.identifier.scopusid | 2-s2.0-85011370890 | - |
dc.identifier.wosid | 000391776500007 | - |
dc.identifier.bibliographicCitation | ANNALS OF LABORATORY MEDICINE, v.37, no.2, pp.147 - 150 | - |
dc.relation.isPartOf | ANNALS OF LABORATORY MEDICINE | - |
dc.citation.title | ANNALS OF LABORATORY MEDICINE | - |
dc.citation.volume | 37 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 147 | - |
dc.citation.endPage | 150 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Medical Laboratory Technology | - |
dc.relation.journalWebOfScienceCategory | Medical Laboratory Technology | - |
dc.subject.keywordPlus | POSTTRANSPLANT LYMPHOPROLIFERATIVE DISORDER | - |
dc.subject.keywordPlus | ORGAN TRANSPLANT RECIPIENTS | - |
dc.subject.keywordPlus | VIRAL LOAD | - |
dc.subject.keywordPlus | ASSAYS | - |
dc.subject.keywordAuthor | Epstein-Barr virus | - |
dc.subject.keywordAuthor | Real-time PCR | - |
dc.subject.keywordAuthor | Quantification | - |
dc.subject.keywordAuthor | Whole blood | - |
dc.subject.keywordAuthor | Performance | - |
dc.identifier.url | https://www.annlabmed.org/journal/view.html?doi=10.3343/alm.2017.37.2.147 | - |
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