Performance of the Real-Q EBV Quantification Kit for Epstein-Barr Virus DNA Quantification in Whole Bloodopen access
- Authors
- Huh, Hee Jae; Eun, Park Jong; Kim, Ji-Youn; Yun, Sun Ae; Lee, Myoung-Keun; Lee, Nam Yong; Kim, Jong-Won; Ki, Chang-Seok
- Issue Date
- Mar-2017
- Publisher
- KOREAN SOC LABORATORY MEDICINE
- Keywords
- Epstein-Barr virus; Real-time PCR; Quantification; Whole blood; Performance
- Citation
- ANNALS OF LABORATORY MEDICINE, v.37, no.2, pp.147 - 150
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- ANNALS OF LABORATORY MEDICINE
- Volume
- 37
- Number
- 2
- Start Page
- 147
- End Page
- 150
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/192389
- DOI
- 10.3343/alm.2017.37.2.147
- ISSN
- 2234-3806
- Abstract
- There has been increasing interest in standardized and quantitative Epstein-Barr virus (EBV) DNA testing for the management of EBV disease. We evaluated the performance of the Real-Q EBV Quantification Kit (BioSewoom, Korea) in whole blood (WB). Nucleic acid extraction and real-time PCR were performed by using the MagNA Pure 96 (Roche Diagnostics, Germany) and 7500 Fast real-time PCR system (Applied Biosystems, USA), respectively. Assay sensitivity, linearity, and conversion factor were determined by using the World Health Organization international standard diluted in EBV-negative WB. We used 81 WB clinical specimens to compare performance of the Real-Q EBV Quantification Kit and artus EBV RG PCR Kit (Qiagen, Germany). The limit of detection (LOD) and limit of quantification (LOQ) for the Real-Q kit were 453 and 750 IU/mL, respectively. The conversion factor from EBV genomic copies to IU was 0.62. The linear range of the assay was from 750 to 106 IU/mL. Viral load values measured with the Real-Q assay were on average 0.54 log(10) copies/mL higher than those measured with the artus assay. The Real-Q assay offered good analytical performance for EBV DNA quantification in WB.
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