Morphology-based assessment of Cd2+ cytotoxicity using microfluidic image cytometry (mu FIC)
DC Field | Value | Language |
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dc.contributor.author | Kim, Min Jung | - |
dc.contributor.author | Lim, Kook Hee | - |
dc.contributor.author | Yoo, Hyun Ju | - |
dc.contributor.author | Rhee, Seog Woo | - |
dc.contributor.author | Yoon, Tae Hyun | - |
dc.date.accessioned | 2024-01-10T02:06:07Z | - |
dc.date.available | 2024-01-10T02:06:07Z | - |
dc.date.issued | 2010-01 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.issn | 1473-0189 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/193935 | - |
dc.description.abstract | Microfluidic systems have significant implications in the field of in vitro cell-based assays since they may allow conventional cellbased assays to be conducted in an automated and high-throughput fashion. In this study, we combined a simple microfluidic cells-onchip system with a morphology-based image cytometric analysis approach for the assessment of Cd2+ induced apoptosis of Chang liver cell line. A simple and efficient in situ monitoring method for quantifying the progress of a cell death event was developed and is presented here. Reasonable agreement of the estimated EC50 value from this study with those from the literature and a close correlation between the observed changes in cell morphology (i.e., circularity) and the amount of reactive oxygen species (ROS) generation confirmed the validity of this morphology-based microfluidic image cytometric (mu FIC) assessment method. We propose this morphology-based mu FIC approach as an easy and efficient way to assess cytotoxicity which can be adapted to high-throughput screening platforms for in vitro cytotoxicity assays as well as drug screening. | - |
dc.format.extent | 3 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Morphology-based assessment of Cd2+ cytotoxicity using microfluidic image cytometry (mu FIC) | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1039/b920890a | - |
dc.identifier.scopusid | 2-s2.0-75749106591 | - |
dc.identifier.wosid | 000274207700002 | - |
dc.identifier.bibliographicCitation | LAB ON A CHIP, v.10, no.4, pp 415 - 417 | - |
dc.citation.title | LAB ON A CHIP | - |
dc.citation.volume | 10 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 415 | - |
dc.citation.endPage | 417 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | LASER-SCANNING CYTOMETRY | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | DEVICE | - |
dc.subject.keywordPlus | CADMIUM | - |
dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2010/LC/b920890a | - |
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