Morphology-based assessment of Cd2+ cytotoxicity using microfluidic image cytometry (mu FIC)
- Authors
- Kim, Min Jung; Lim, Kook Hee; Yoo, Hyun Ju; Rhee, Seog Woo; Yoon, Tae Hyun
- Issue Date
- Jan-2010
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- LAB ON A CHIP, v.10, no.4, pp 415 - 417
- Pages
- 3
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- LAB ON A CHIP
- Volume
- 10
- Number
- 4
- Start Page
- 415
- End Page
- 417
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/193935
- DOI
- 10.1039/b920890a
- ISSN
- 1473-0197
1473-0189
- Abstract
- Microfluidic systems have significant implications in the field of in vitro cell-based assays since they may allow conventional cellbased assays to be conducted in an automated and high-throughput fashion. In this study, we combined a simple microfluidic cells-onchip system with a morphology-based image cytometric analysis approach for the assessment of Cd2+ induced apoptosis of Chang liver cell line. A simple and efficient in situ monitoring method for quantifying the progress of a cell death event was developed and is presented here. Reasonable agreement of the estimated EC50 value from this study with those from the literature and a close correlation between the observed changes in cell morphology (i.e., circularity) and the amount of reactive oxygen species (ROS) generation confirmed the validity of this morphology-based microfluidic image cytometric (mu FIC) assessment method. We propose this morphology-based mu FIC approach as an easy and efficient way to assess cytotoxicity which can be adapted to high-throughput screening platforms for in vitro cytotoxicity assays as well as drug screening.
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