Chlamydomonas reinhardtii dZL 균주의 광도가 세포 생장과 카로티노이드 생산량에 미치는 영향 연구

Abstract

Microalgae, as photosynthetic organisms, possess the ability to produce a diverse array of bioactive compounds. This study focused on the transformant Chlamydomonas reinhardtii dZL and subjected it to cultivation under varying light intensities (60, 120, 180, and 240 μmol/m2/s). Our aim was to assess the impact of light intensity on both microalgal biomass and carotenoid production. The cultivation took place in 80 mL bubble column photobioreactors, specifically the Multi-cultivator. Notably, the culture exposed to 240 μmol/m2/s exhibited the most rapid cell growth, surpassing even the cell concentration achieved at 180 μmol/m2/s by day 8. A detailed analysis of the specific irradiance rate over time unequivocally revealed a sharp decline in growth rates when the rate fell below 2 × 10-10 μmol/cell/s. Although the culture with 60 μmol/m2/s yielded the highest carotenoid content (1.2% of dry weight), the culture exposed to 240 μmol/m2/s recorded the highest carotenoid concentration at 8.9 mg/L owing to its higher biomass. Our findings reveal the critical importance of maintaining a specific irradiance rate above 2 × 10-10 μmol/cell/s to enhance biomass and carotenoid productivity. This study lays the groundwork for defining optimal light intensity conditions applicable to mass culture systems, with the objective of augmenting C. reinhardtii biomass and optimizing carotenoid productivity.