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Protein purification, crystallization, and structure determination of transcription factor YhaJ in complex with DNT metabolitesopen access
- Authors
- Kim, Myeongbin; Kang, Ryun; Jeon, Tae Jin; Ryu, Seong Eon
- Issue Date
- Jun-2024
- Publisher
- Cell Press
- Keywords
- Biotechnology and bioengineering; Protein expression and purification; X-ray Crystallography
- Citation
- STAR Protocols, v.5, no.2, pp 1 - 17
- Pages
- 17
- Indexed
- SCOPUS
ESCI
- Journal Title
- STAR Protocols
- Volume
- 5
- Number
- 2
- Start Page
- 1
- End Page
- 17
- ISSN
- 2666-1667
2666-1667
- Abstract
- The microbial transcription factor YhaJ responds to 2,4-dinitrotoluene (DNT) derivatives. Here, we describe steps for overexpression and purification of the protein, characterization for the binding of a DNT derivative methylhydroquinone, and crystallization by using a random seeding technique. We then detail procedures for structure determination by employing the crystal-twin resolving processes. This protocol can also be performed using other DNT derivatives. For complete details on the use and execution of this protocol, please refer to Kim et al.
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Related Researcher
- Ryu, Seong Eon
- COLLEGE OF ENGINEERING (DEPARTMENT OF BIOENGINEERING)