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Portable platform for measuring amyloid beta 42/40 ratio via photooxidation-induced fluorescence amplification
- Authors
- Ko, Sanghag; Bae, Hyunjun; Kim, Daewon; Lee, Yeonju; Choi, Isaac; Lee, Dain; Choi, Dong hwan; Kim, Hyung Chul; Sohn, Seo Young; Jeong, Yohan; Chung, Seok; Jung, Young Hee
- Issue Date
- Aug-2025
- Publisher
- Royal Society of Chemistry
- Keywords
- Polypropylene; Resorufin; Amyloid Beta Protein; Amyloid Beta-peptides; Amyloid Beta-protein (1-40); Amyloid Beta-protein (1-42); Peptide Fragments; Amplification; Biomarkers; Blood; Brain; Cost Effectiveness; Neurodegenerative Diseases; Patient Treatment; Positron Emission Tomography; Alzheimers Disease; Amyloid Betas; Cognitive Decline; Cognitive Impairment; Disease Biomarker; Emission Tomography; Health Condition; Measurements Of; Positron Emission; Single Molecule; Cerebrospinal Fluid; Amyloid Beta Protein[1-40]; Amyloid Beta Protein[1-42]; Apolipoprotein E4; Polypropylene; Resorufin; Amyloid Beta Protein; Amyloid Beta-protein (1-42); Peptide Fragment; Adult; Aged; Area Under The Curve; Article; Brain; Cerebrospinal Fluid; Clinical Article; Clinical Dementia Rating Sum Of Boxes; Cognitive Defect; Controlled Study; Cost Effectiveness Analysis; Daily Life Activity; Diagnostic Test Accuracy Study; Female; Fluorescence; Human; Immunoassay; Male; Mild Cognitive Impairment; Mini Mental State Examination; Nuclear Magnetic Resonance Imaging; Photooxidation; Positron Emission Tomography; Protein Blood Level; Signal Detection; Single Molecule Immunoassay; Alzheimer Disease; Blood; Diagnosis; Oxidation Reduction Reaction; Photochemistry; Alzheimer Disease; Amyloid Beta-peptides; Cognitive Dysfunction; Fluorescence; Humans; Immunoassay; Oxidation-reduction; Peptide Fragments; Photochemical Processes
- Citation
- Lab on a Chip, v.25, no.16, pp 3993 - 4001
- Pages
- 9
- Indexed
- SCIE
SCOPUS
- Journal Title
- Lab on a Chip
- Volume
- 25
- Number
- 16
- Start Page
- 3993
- End Page
- 4001
- ISSN
- 1473-0197
1473-0189
- Abstract
- Alzheimer's disease (AD) is a serious health condition that exacerbates with age. Among various AD biomarkers, the measurement of the A beta 42/40 ratio (i.e., ratio of A beta 42 and A beta 40 concentrations) has garnered prominence for the early identification of AD patients and the development of disease-modifying treatments. Approaches such as positron emission tomography examinations and biomarker measurements in cerebrospinal fluid and blood plasma face constraints related to expense and procedural complexity. To address these issues, we developed and evaluated the efficacy of a portable platform for detecting amyloid beta (A beta) 40 and 42 in plasma, utilizing photooxidation-induced fluorescence amplification (PIFA). We conducted a comparative analysis of A beta 42/40 measurements between the PIFA and single-molecule immunoassay (SiMoA) platforms. By measuring 38 cases of subjective cognitive decline (SCD), 24 cases of amnestic mild cognitive impairment (aMCI), and 46 cases of AD dementia samples, we observed a significant difference in A beta 42/40 ratios between the SCD and aMCI groups. The PIFA platform demonstrated an area under the curve compared to that of the SiMoA platform, which is currently the most precise method for A beta 42/40 ratio measurement. Consequently, the PIFA platform presents a viable cost-effective tool for detecting the A beta 42/40 ratio.
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