Generation of Multilayered 3D Structures of HepG2 Cells Using a Bio-printing Technique
- Authors
- Jeon, Hyeryeon; Kang, Kyojin; Park, Su A.; Kim, Wan Doo; Paik, Seung Sam; Lee, Sang-Hun; Jeong, Jaemin; Choi, Dongho
- Issue Date
- Jan-2017
- Publisher
- EDITORIAL OFFICE GUT & LIVER
- Keywords
- Hep G2 cell; Printing; three-dimensional
- Citation
- GUT AND LIVER, v.11, no.1, pp.121 - 128
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- GUT AND LIVER
- Volume
- 11
- Number
- 1
- Start Page
- 121
- End Page
- 128
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/21230
- DOI
- 10.5009/gnl16010
- ISSN
- 1976-2283
- Abstract
- Background/Aims: Chronic liver disease is a major widespread cause of death, and whole liver transplantation is the only definitive treatment for patients with end-stage liver diseases. However, many problems, including donor shortage, surgical complications and cost, hinder their usage. Recently, tissue-engineering technology provided a potential breakthrough for solving these problems. Three-dimensional (3D) printing technology has been used to mimic tissues and organs suitable for transplantation, but applications for the liver have been rare. Methods: A 3D bioprinting system was used to construct 3D printed hepatic structures using alginate. HepG2 cells were cultured on these 3D structures for 3 weeks and examined by fluorescence microscopy, histology and immunohistochemistry. The expression of liver specific markers was quantified on days 1, 7, 14, and 21. Results: The cells grew well on the alginate scaffold, and liver-specific gene expression increased. The cells grew more extensively in 3D culture than two-dimensional culture and exhibited better structural aspects of the liver, indicating that the 3D bioprinting method recapitulates the liver architecture. Conclusions: The 3D bioprinting of hepatic structures appears feasible. This technology may become a major tool and provide a bridge between basic science and the clinical challenges for regenerative medicine of the liver.
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