Elevated STC‑1 augments the invasiveness of triple‑negative breast cancer cells through activation of the JNK/c‑Jun signaling pathway
- Authors
- Han, Jeonghun; Jeon, Myeongjin; Shin, Incheol; Kim, Sangmin
- Issue Date
- Sep-2016
- Publisher
- SPANDIDOS PUBL LTD
- Keywords
- stanniocalcin-1; prognosis; triple-negative breast cancer; cell invasion; JNK
- Citation
- ONCOLOGY REPORTS, v.36, no.3, pp.1764 - 1771
- Indexed
- SCIE
SCOPUS
- Journal Title
- ONCOLOGY REPORTS
- Volume
- 36
- Number
- 3
- Start Page
- 1764
- End Page
- 1771
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/22213
- DOI
- 10.3892/or.2016.4977
- ISSN
- 1021-335X
- Abstract
- Stanniocalcin-1 (STC-1), a secreted glycoprotein, is highly expressed in a variety of human malignancies. However, the role of STC-1 has not been fully elucidated in breast cancer cells. Here, we investigated whether STC-1 acts as a prognostic factor in triple-negative breast cancer (TNBC) patients, and we explored the cellular mechanism in breast cancer cells. The level of STC-1 expression was directly associated with the relapse-free and overall survival of basal-type breast cancer patients. Breast cancer patients with a high level of STC-1 had poor prognosis. In addition, our results showed that the level of STC-1 expression was markedly higher in TNBC than in non-TNBC cells. Invasiveness of the TNBC cells was also significantly increased in response to recombinant human STC-1 treatment. In contrast, the invaded cell numbers were completely decreased by STC-1 siRNA overexpression in the Hs578T and MDA-MB-231 TNBC cells. Our results showed that the phosphorylation of c-Jun N-terminal protein kinase (JNK) and c-Jun was increased after STC-1 treatment but not the phosphorylation of ERK and p38 MAPKs in the Hs578T and MDA-MB-231 TNBC cells. Furthermore, expression of one invasion-related gene MMP-9, was increased by STC-1 treatment. STC-1-induced MMP-9 expression was suppressed by SP600125 (a JNK inhibitor) in the Hs578T cells. STC-1-induced cell invasiveness was also inhibited by SP600125. Taken together, we demonstrated that aberrant STC-1 expression is associated with poor prognosis and stimulates the invasiveness of TNBC cells through the JNK/c-Jun-dependent signaling pathway.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 자연과학대학 > 서울 생명과학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/22213)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.