Programmable Molecular Scissors: Applications of a New Tool for Genome Editing in Biotechopen access
- Authors
- Saha, Subbroto Kumar; Saikot, Forhad Karim; Rahman, Md. Shahedur; Jamal, Mohammad Abu Hena Mostofa; Rahman, S. M. Khaledur; Islam, S. M. Riazul; Kim, Ki-Hyun
- Issue Date
- Mar-2019
- Publisher
- CELL PRESS
- Keywords
- CRISPR-Cas9; DSB; genome editing; HDR; NHEJ; nucleases; off-target mutagenesis; ssODNs; TALENs; ZFNs
- Citation
- MOLECULAR THERAPY-NUCLEIC ACIDS, v.14, pp.212 - 238
- Indexed
- SCIE
SCOPUS
- Journal Title
- MOLECULAR THERAPY-NUCLEIC ACIDS
- Volume
- 14
- Start Page
- 212
- End Page
- 238
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/2245
- DOI
- 10.1016/j.omtn.2018.11.016
- ISSN
- 2162-2531
- Abstract
- Targeted genome editing is an advanced technique that enables precise modification of the nucleic acid sequences in a genome. Genome editing is typically performed using tools, such as molecular scissors, to cut a defined location in a specific gene. Genome editing has impacted various fields of biotechnology, such as agriculture; biopharmaceutical production; studies on the structure, regulation, and function of the genome; and the creation of transgenic organisms and cell lines. Although genome editing is used frequently, it has several limitations. Here, we provide an overview of well-studied genome-editing nucleases, including single-stranded oligodeoxynucleotides (ssODNs), transcription activator-like effector nucleases (TALENs), zinc-finger nucleases (ZFNs), and CRISPR-Cas9 RNA-guided nucleases (CRISPR-Cas9). To this end, we describe the progress toward editable nuclease-based therapies and discuss the minimization of off-target mutagenesis. Future prospects of this challenging scientific field are also discussed.
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Collections - 서울 공과대학 > 서울 건설환경공학과 > 1. Journal Articles
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