Low doses of PEG-coated gold nanoparticles sensitize solid tumors to cold plasma by blocking the PI3K/AKT-driven signaling axis to suppress cellular transformation by inhibiting growth and EMT
- Authors
- Kaushik, Nagendra Kumar; Kaushik, Neha; Yoo, Ki Chun; Uddin, Nizam; Kim, Ju Sung; Lee, Su Jae; Choi, Eun Ha
- Issue Date
- May-2016
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Cold plasma; Gold nanoparticles; Solid cancers; Stemness; Glioma-like stem cells; Epithelial-mesenchymal transition
- Citation
- BIOMATERIALS, v.87, pp.118 - 130
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMATERIALS
- Volume
- 87
- Start Page
- 118
- End Page
- 130
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/23092
- DOI
- 10.1016/j.biomaterials.2016.02.014
- ISSN
- 0142-9612
- Abstract
- Metastasis, the primary cause of tumor cell transformation, is often activated during cancer invasion and progression and is associated with poor therapeutic outcomes. The effects of combined treatments that included PEG-coated gold nanoparticles (GNP) and cold plasma on epithelial-mesenchymal transition (EMT) and the maintenance of cancer stem cells (CSC) have not been described so far. Here, we report that co-treatment with GNP and cold plasma inhibited proliferation in cancer cells by abolishing the activation of the PI3K/AKT signaling axis. In addition, co-treatment reversed EMT in solid tumor cells by reducing the secretion of a number of proteins, resulting in the upregulation of epithelial markers such as E-cadherin along with down-regulation of N-Cadherin, Slug and Zeb-1. The inhibition of the PI3K/AKT pathway and the reversal of EMT by co-treatment prevented tumor cells growth in solid tumors. Furthermore, we show that GNP and plasma also suppresses tumor growth by decreasing mesenchymal markers in tumor xenograft mice models. Importantly, co-treatment resulted in a substantial decrease in sphere formation and the self-renewal capacity of glioma-like stem cells. Together, these results indicate a direct link between a decrease of EMT and an increase in cell death in solid tumors following co treatment with cold plasma and GNP.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 서울 자연과학대학 > 서울 생명과학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/23092)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.