Modulation of Charge Density of Cationic Conjugated Polyelectrolytes for Improving the FRET‐Induced Sensory Signal with Enhanced On/Off Ratio
- Authors
- Jeong, Ji-Eun; Jung, In Hwan; Kim, Boram; Le, Van Sang; Woo, Han Young
- Issue Date
- Feb-2016
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- conjugated polyelectrolytes; fluorescence resonance energy transfer; ionic density; on; off ratio; water-solubility
- Citation
- MACROMOLECULAR CHEMISTRY AND PHYSICS, v.217, no.3, pp.459 - 466
- Indexed
- SCIE
SCOPUS
- Journal Title
- MACROMOLECULAR CHEMISTRY AND PHYSICS
- Volume
- 217
- Number
- 3
- Start Page
- 459
- End Page
- 466
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/23955
- DOI
- 10.1002/macp.201500304
- ISSN
- 1022-1352
- Abstract
- Three types of cationic polyfluorenes with a different number of ionic groups are synthesized to modulate their solubility in water and the interactions with anionic oligonucleotide probes (DNA-C). Increasing the charge density improves the water-solubility of conjugated polyelectrolytes (CPEs) but decreases its optical antenna effect in the electrostatic sensory platform (CPE/DNA-C), resulting in low fluorescence resonance energy transfer (FRET) signal. With increasing charge density in CPE, the concentration of conjugated units in CPE/DNA-C decreases at [+]:[-] = 1:1, after which little driving force is expected to bring positive CPEs and negative DNA-C into close proximity for efficient FRET. CPF2 with 2 ionic groups (per repeat unit) and hydrophilic polyether side-chains exhibits good water-solubility and a higher FRET signal with an enhanced signal on/off ratio. In addition, the charge density in CPEs modulates the range of sensory responses by forming a tighter or weaker electrostatic CPE/DNA-C complex, which perturbs the probe-target binding interactions.
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