Identification of RNA-Binding Proteins by RNA Ligand-based cDNA Expression Library Screening
- Authors
- Kim, Min Young; Lee, Jong Joo; Kim, Chul Geun
- Issue Date
- Jan-2016
- Publisher
- Bio-protocol LLC
- Citation
- bio-protocol, v.6, no.2, pp.1 - 8
- Indexed
- OTHER
- Journal Title
- bio-protocol
- Volume
- 6
- Number
- 2
- Start Page
- 1
- End Page
- 8
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/24043
- DOI
- 10.21769/BioProtoc.1715
- ISSN
- 2331-8325
- Abstract
- We previously reported when a portion of the Requiem (REQ/DPF2) messenger ribonucleic acid (mRNA) 3’ untranslated region (3’UTR), referred to as G8, was overexpressed in K562 cells, β-globin expression was induced, suggesting that the 3’UTR of REQ mRNA plays a physiological role (Kim et al., 2014). To identify trans-acting factors that bind to the REQ 3’UTR, we describe the RNA ligand based cDNA expression library screening method. This protocol could be adapted to detect specific RNA-protein interactions. Following this method, we identified six positive clones in the initial round of screening and four pure clones after sib-screening. This protocol was originally published in Kim et al. (2014).
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