Three-dimensional (3D) printing of mouse primary hepatocytes to generate 3D hepatic structure
DC Field | Value | Language |
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dc.contributor.author | Kim, Yohan | - |
dc.contributor.author | Kang, Kyojin | - |
dc.contributor.author | Jeong, Jaemin | - |
dc.contributor.author | Paik, Seung Sam | - |
dc.contributor.author | Kim, Ji Sook | - |
dc.contributor.author | Park, Su A. | - |
dc.contributor.author | Kim, Wan Doo | - |
dc.contributor.author | Park, Jisun | - |
dc.contributor.author | Choi, Dongho | - |
dc.date.accessioned | 2021-07-30T04:58:36Z | - |
dc.date.available | 2021-07-30T04:58:36Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2017-02 | - |
dc.identifier.issn | 2288-6575 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/2478 | - |
dc.description.abstract | Purpose: The major problem in producing artificial livers is that primary hepatocytes cannot be cultured for many days. Recently, 3-dimensional (3D) printing technology draws attention and this technology regarded as a useful tool for current cell biology. By using the 3D bio-printing, these problems can be resolved. Methods: To generate 3D bio-printed structures (25 mm x 25 mm), cells-alginate constructs were fabricated by 3D bio-printing system. Mouse primary hepatocytes were isolated from the livers of 6-8 weeks old mice by a 2-step collagenase method. Samples of 4 x 10(7) hepatocytes with 80%-90% viability were printed with 3% alginate solution, and cultured with well-defined culture medium for primary hepatocytes. To confirm functional ability of hepatocytes cultured on 3D alginate scaffold, we conducted quantitative real-time polymerase chain reaction and immunofluorescence with hepatic marker genes. Results: Isolated primary hepatocytes were printed with alginate. The 3D printed hepatocytes remained alive for 14 days. Gene expression levels of Albumin, HNF-4 alpha and Foxa3 were gradually increased in the 3D structures. Immunofluorescence analysis showed that the primary hepatocytes produced hepatic-specific proteins over the same period of time. Conclusion: Our research indicates that 3D bio-printing technique can be used for long-term culture of primary hepatocytes. It can therefore be used for drug screening and as a potential method of producing artificial livers. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SURGICAL SOCIETY | - |
dc.title | Three-dimensional (3D) printing of mouse primary hepatocytes to generate 3D hepatic structure | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Paik, Seung Sam | - |
dc.contributor.affiliatedAuthor | Choi, Dongho | - |
dc.identifier.doi | 10.4174/astr.2017.92.2.67 | - |
dc.identifier.scopusid | 2-s2.0-85014895125 | - |
dc.identifier.wosid | 000393445500002 | - |
dc.identifier.bibliographicCitation | ANNALS OF SURGICAL TREATMENT AND RESEARCH, v.92, no.2, pp.67 - 72 | - |
dc.relation.isPartOf | ANNALS OF SURGICAL TREATMENT AND RESEARCH | - |
dc.citation.title | ANNALS OF SURGICAL TREATMENT AND RESEARCH | - |
dc.citation.volume | 92 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 67 | - |
dc.citation.endPage | 72 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002192963 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.subject.keywordPlus | EXTRACELLULAR-MATRIX | - |
dc.subject.keywordPlus | CELL-LINES | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ALGINATE | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordAuthor | Hepatocytes | - |
dc.subject.keywordAuthor | Three-dimensional printing | - |
dc.subject.keywordAuthor | Culture | - |
dc.subject.keywordAuthor | Maintenance | - |
dc.identifier.url | https://astr.or.kr/DOIx.php?id=10.4174/astr.2017.92.2.67 | - |
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