Three-dimensional (3D) printing of mouse primary hepatocytes to generate 3D hepatic structureopen access
- Authors
- Kim, Yohan; Kang, Kyojin; Jeong, Jaemin; Paik, Seung Sam; Kim, Ji Sook; Park, Su A.; Kim, Wan Doo; Park, Jisun; Choi, Dongho
- Issue Date
- Feb-2017
- Publisher
- KOREAN SURGICAL SOCIETY
- Keywords
- Hepatocytes; Three-dimensional printing; Culture; Maintenance
- Citation
- ANNALS OF SURGICAL TREATMENT AND RESEARCH, v.92, no.2, pp.67 - 72
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- ANNALS OF SURGICAL TREATMENT AND RESEARCH
- Volume
- 92
- Number
- 2
- Start Page
- 67
- End Page
- 72
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/2478
- DOI
- 10.4174/astr.2017.92.2.67
- ISSN
- 2288-6575
- Abstract
- Purpose: The major problem in producing artificial livers is that primary hepatocytes cannot be cultured for many days. Recently, 3-dimensional (3D) printing technology draws attention and this technology regarded as a useful tool for current cell biology. By using the 3D bio-printing, these problems can be resolved. Methods: To generate 3D bio-printed structures (25 mm x 25 mm), cells-alginate constructs were fabricated by 3D bio-printing system. Mouse primary hepatocytes were isolated from the livers of 6-8 weeks old mice by a 2-step collagenase method. Samples of 4 x 10(7) hepatocytes with 80%-90% viability were printed with 3% alginate solution, and cultured with well-defined culture medium for primary hepatocytes. To confirm functional ability of hepatocytes cultured on 3D alginate scaffold, we conducted quantitative real-time polymerase chain reaction and immunofluorescence with hepatic marker genes. Results: Isolated primary hepatocytes were printed with alginate. The 3D printed hepatocytes remained alive for 14 days. Gene expression levels of Albumin, HNF-4 alpha and Foxa3 were gradually increased in the 3D structures. Immunofluorescence analysis showed that the primary hepatocytes produced hepatic-specific proteins over the same period of time. Conclusion: Our research indicates that 3D bio-printing technique can be used for long-term culture of primary hepatocytes. It can therefore be used for drug screening and as a potential method of producing artificial livers.
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