Isolation of mesenchymal stem-like cells in meningioma specimens
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lim, Hyo-Yeol | - |
dc.contributor.author | Kim, Kyung Min | - |
dc.contributor.author | Kim, Bo Kyung | - |
dc.contributor.author | Shim, Jin-Kyoung | - |
dc.contributor.author | Lee, Ji-Hyun | - |
dc.contributor.author | Huh, Yong-Min | - |
dc.contributor.author | Kim, Se-Hoon | - |
dc.contributor.author | Kim, Eui-Hyun | - |
dc.contributor.author | Park, Eun-Kyung | - |
dc.contributor.author | Shim, Kyu-Won | - |
dc.contributor.author | Chang, Jong Hee | - |
dc.contributor.author | Kim, Dong-Seok | - |
dc.contributor.author | Kim, Sun Ho | - |
dc.contributor.author | Hong, Yong-Kil | - |
dc.contributor.author | Lee, Su-Jae | - |
dc.contributor.author | Kang, Seok-Gu | - |
dc.date.accessioned | 2021-08-02T18:54:16Z | - |
dc.date.available | 2021-08-02T18:54:16Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2013-10 | - |
dc.identifier.issn | 1019-6439 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/26629 | - |
dc.description.abstract | Cells resembling bone marrow mesenchymal stem cells (BM-MSCs) have been isolated from glioma specimens; however, little is known about the existence of mesenchymal stem-like cells (MSLCs) in meningioma. Here, we hypothesized that cells similar to BM-MSCs exist in meningioma specimens and sought to investigate whether these putative meningioma stroma MSLCs (MS-MSLCs) could be isolated. To this end, we cultured fresh meningioma specimens using the same protocols as used previously to isolate BM-MSC. Cultured cells were analyzed for surface markers associated with BM-MSCs by fluorescence-activated cell sorting (FACS) and candidate cells were exposed to mesenchymal differentiation conditions. Possible locations of MS-MSLCs were determined by immunohistochemical analysis of sections of meningioma specimens. Spindle-shaped and, adherent cells similar to BM-MSCs were isolated in 2 of 20 meningioma specimens. FACS analysis showed that the surface markers of MS-MSLCs were similar to those of BM-MSCs and the chosen cells demonstrated an ability to differentiate into osteogenic, adipogenic and chondrogenic cells. The tumorigenicity of MS-MSLCs was tested by injection of these cells into the brain of athymic nude mice; no tumors were subsequently discovered. Immunohistochemical analyses indicated that CD105(+) cells were closely associated with endothelial cells and pericytes in meningioma specimens. Our results established for the first time that cells similar to BM-MSCs exist in meningioma specimens. These cells, termed MS-MSLCs, could be one component of the meningioma cellular microenvironment. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.title | Isolation of mesenchymal stem-like cells in meningioma specimens | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Su-Jae | - |
dc.identifier.doi | 10.3892/ijo.2013.2053 | - |
dc.identifier.scopusid | 2-s2.0-84882395234 | - |
dc.identifier.wosid | 000324603900036 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF ONCOLOGY, v.43, no.4, pp.1260 - 1268 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF ONCOLOGY | - |
dc.citation.title | INTERNATIONAL JOURNAL OF ONCOLOGY | - |
dc.citation.volume | 43 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1260 | - |
dc.citation.endPage | 1268 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | CENTRAL-NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | CANCER METASTASIS | - |
dc.subject.keywordPlus | GLIOMA SPECIMENS | - |
dc.subject.keywordPlus | ARACHNOID CELLS | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | BRAIN CANCER | - |
dc.subject.keywordPlus | TUMOR-CELLS | - |
dc.subject.keywordPlus | BONE-MARROW | - |
dc.subject.keywordPlus | STROMA | - |
dc.subject.keywordPlus | MICROENVIRONMENT | - |
dc.subject.keywordAuthor | meningioma | - |
dc.subject.keywordAuthor | meningioma stroma | - |
dc.subject.keywordAuthor | mesenchymal stem-like cells | - |
dc.subject.keywordAuthor | microenvironment | - |
dc.subject.keywordAuthor | perivascular area | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.