Isolation of mesenchymal stem-like cells in meningioma specimens
- Authors
- Lim, Hyo-Yeol; Kim, Kyung Min; Kim, Bo Kyung; Shim, Jin-Kyoung; Lee, Ji-Hyun; Huh, Yong-Min; Kim, Se-Hoon; Kim, Eui-Hyun; Park, Eun-Kyung; Shim, Kyu-Won; Chang, Jong Hee; Kim, Dong-Seok; Kim, Sun Ho; Hong, Yong-Kil; Lee, Su-Jae; Kang, Seok-Gu
- Issue Date
- Oct-2013
- Publisher
- SPANDIDOS PUBL LTD
- Keywords
- meningioma; meningioma stroma; mesenchymal stem-like cells; microenvironment; perivascular area
- Citation
- INTERNATIONAL JOURNAL OF ONCOLOGY, v.43, no.4, pp.1260 - 1268
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF ONCOLOGY
- Volume
- 43
- Number
- 4
- Start Page
- 1260
- End Page
- 1268
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/26629
- DOI
- 10.3892/ijo.2013.2053
- ISSN
- 1019-6439
- Abstract
- Cells resembling bone marrow mesenchymal stem cells (BM-MSCs) have been isolated from glioma specimens; however, little is known about the existence of mesenchymal stem-like cells (MSLCs) in meningioma. Here, we hypothesized that cells similar to BM-MSCs exist in meningioma specimens and sought to investigate whether these putative meningioma stroma MSLCs (MS-MSLCs) could be isolated. To this end, we cultured fresh meningioma specimens using the same protocols as used previously to isolate BM-MSC. Cultured cells were analyzed for surface markers associated with BM-MSCs by fluorescence-activated cell sorting (FACS) and candidate cells were exposed to mesenchymal differentiation conditions. Possible locations of MS-MSLCs were determined by immunohistochemical analysis of sections of meningioma specimens. Spindle-shaped and, adherent cells similar to BM-MSCs were isolated in 2 of 20 meningioma specimens. FACS analysis showed that the surface markers of MS-MSLCs were similar to those of BM-MSCs and the chosen cells demonstrated an ability to differentiate into osteogenic, adipogenic and chondrogenic cells. The tumorigenicity of MS-MSLCs was tested by injection of these cells into the brain of athymic nude mice; no tumors were subsequently discovered. Immunohistochemical analyses indicated that CD105(+) cells were closely associated with endothelial cells and pericytes in meningioma specimens. Our results established for the first time that cells similar to BM-MSCs exist in meningioma specimens. These cells, termed MS-MSLCs, could be one component of the meningioma cellular microenvironment.
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